x a CRIPPLED: OUEREN 261 
largest, and, after removal, made a ball of wax 
§ in. in diameter. Only about one-third of this 
quantity had exuded from the base of the 4th 
segment ; the amount at the base of the 3rd segment 
was equal to the amount at the base of the 4th, 
while the quantity at the base of the 6th segment 
was very much less, and I computed it to be about 
one-tenth of what had oozed from the base of the 
3rd segment. The 2nd segment on the left and the 
under side of the abdomen on the left were greasy, 
but they bore no wax. 
I mention the quantities of wax as accurately as 
I was able to estimate them, because I think that 
they probably represent the proportion of wax that 
exudes from between each segment in a normal 
queen. It will have been noticed that the quantity 
given off between the 4th and 5th segments 
was greater than the total quantity from all the 
other segments. This is the part of the abdomen that 
is most easily reached by the brushes on the hind 
metatarsi, which no doubt are used to remove the 
wax, for in a queen that had one of her hind meta- 
tarsi slightly paralysed the brush on it was covered 
with wax. 
The fact that my A queen, having lost her 
left hind tibia, had been able to keep the right side 
of her abdomen clean shows that the wax is not 
removed from the metatarsal brush by the tibial 
comb and passed on to the corbicula like the pollen. 
How, then, was it removed from the metatarsal brush 
in this case? At the apical end of the metatarsal 
