303 



There is a specimen in which the injury in the third instar 

 is represented by a crust across the basal parts, but in the fourth, 

 fifth, and the imago, there is nothing to note except reduced 

 size. Another specimen is similar, but that the leg is much 

 reduced in the fourth and fifth instars, with abbreviation of 

 the tibia and tarsus as in ordinary regeneration, and but little 

 diminution in size in the imago. In another similar specimen 

 the fifth instar is very much like specimens where regeneration 

 is complete, but the imago has the femur, tibia, and tarsus of 

 little more than half the normal length. 



Another specimen, with a less severe injury in the second 

 instar, shows considerable reduction in size in the fourth instar, 

 very little in the fifth, and an imago with a barely appreciable 

 reduction in size. 



Related to these last noted specimens, are some sixty-four 

 preparations, in which the larval leg, generally in the last instar, 

 was more or less crushed, without actual wound. 



In twenty-six of these the injury has had little or no result, 

 the imaginai legs being perfect, and with trifling reduction of 

 size in two or three instances. In thirty-three, on the other 

 hand, the leg is either wanting or much reduced, in the remainder 

 there is a reduction in the number of tarsal joints in an otherwise 

 more or less well-developed leg. 



Amongst these specimens are twelve that show more or less 

 duplication of parts. Omitting the twenty-six examples in which 

 the supposed injury did not, in fact, take place, these duplications 

 are twelve in thirty-eight, or over 30 per cent. 



Amongst the amputations only one (fig. 23) shows anything 

 that resembles duplication, and in this there is only an excess 

 of claws, which is not very unusual, and can hardly be called 

 duplication, as it seems to be a result of the very strong deter- 

 mination there seems to be that the claws shall be reproduced, 

 no matter how primitive the other parts may be. 



As the duplications observed were the result of operations 

 on the last larval instar, it is probable that duplications of a 

 more organised character, and more like a portion of an ordinary 

 limb, would result from experiments in earlier instars. 



Some of the more marked of these specimens are represented 

 in figs. 30, 31, 32, 34, 35, 36, zi, 38, 39. '1"^ 40- 



