No. I.] FILOSE ACTIVITY IN METAZOAN EGGS. 



connections and other spinnings have been retained, both in 

 starfish and in sea-urchin eggs for three years. 



Thus in Fig. 3 a four-cell stage in the common echinus of 

 Roscoff shows filaments passing from cell to cell. These are 

 drawn considerably thicker than the actual threads seen, but 

 otherwise represent them truly. The figure is a surface view 

 camera drawing with oc. 8, obj. 2 mm., tube 160 mm., helped 



Fig. 4. 



out with ocs. 12 and 18. On the left, above, a series of eleva- 

 tions from one cell seemed to be the remnants of tufts of fila- 

 ments, while the granular matter, imperfectly represented here, 

 partly covering the cleavage pore, appeared to be the same as 

 Hammar's ectoplasmic layer.^ 



There can be no doubt that these filamentous intercellular 

 connections are the preserved remnants of the active filose 

 threads of the living Q.gg. 



Some eggs of Amphioxus killed in corrosive acetic and 

 stained in Orth's new lithium carmine, as well as some killed 



1 Andrews, E. A., " Hammar's Ectoplasmic Layer," American Naturalist. 

 December, 1897. 



