2 ANDREWS. [Vol. II. 



the eggs of several other animals examined, and thus to extend 

 the known occurrence of filose activities amongst Metazoa so 

 widely that its importance seems strengthened and the proba- 

 bility of its still wider existence increased. 



The first eggs examined, the frog's eggs in cleavage and gas- 

 trula stages, yielded when studied alive only the amoeboid 

 movement described by Roux; certain sections, however, showed 

 intercellular connections that lead me to expect filose phenom- 

 ena to be present here. A large number of sections of cleavage 

 and larval stages in various frogs and in the salamander y4;//(^/j/- 

 stoma ptinctattim were carefully studied. In many sections of the 

 latter animal, prepared and kindly loaned to me by Prof. C. B. 

 Wilson of Westfield, Mass., as well as in certain frog's eggs, un- 

 doubted intercellular connections exist ; but as their filose nature 

 is not demonstrated, they will be but briefly noticed here. In 

 the larva when the medullary folds are closed and the split meso- 

 blast nearly fused on the ventral side, intercellular connections 

 were seen between the large yolk cells, between mesoderm and 

 mesenchyme cells, between the ectoderm cells on opposite sides 

 of the nerve tube, between the ectoderm and mesoderm, and 

 between the entoderm and mesoderm; in fact, cells in all germ 

 layers and in each layer connect with those in another layer 

 and with those in the same. Eliminating the deceitful appear- 

 ances produced by coagulation of liquids between cells, by 

 coagulation of fixative, by vacuolization and shrinkage of the 

 superficial parts of cells, by the throwing off of pellicles, by the 

 edges of drops and vesicles, and by fragments of vitelline mem- 

 brane, as well as by scratches upon slide and cover glass, there 

 still remained the above intercellular connections of undoubted 

 protoplasm. These varied from fine filaments to broad bridges, 

 and were either clear or contained some of the pigment granules 

 of the egg. That they were filose in nature was indicated by 

 their proportions and mode of origin and insertion; yet there 

 was not decisive evidence that they were not produced in other 

 ways either in the normal egg or in the egg when dying. • 



The next eggs, those of the annelid Serpula, were only exam- 

 ined alive and showed filaments passing out from the surface of 

 the egg toward and to the membrane, both before and after the 



