130 FOOT AND STROBELL. [Vol. II. 



by our combined efforts, we unite in bearing the responsibility 

 of their pubhcation, — parts of the following paper being 

 written by each of us. Owing to the difficulties of obtaining 

 large numbers of these eggs at definite stages of development, 

 it will require both time and patience to make a comparative 

 study of much value. In the present paper we shall give a 

 brief account of such of our results as we can support with 

 photomicrographs; and I shall also give certain results obtained 

 from a further study of the deutoplasm of these eggs, some data 

 bearingr on the formation of the vesicles between the first and 

 second maturation spindles, and a few notes on shrinkage. 



With orange-methyl green I had differentiated the following 

 structures in the cytoplasm (6), the network and archoplasm 

 (polar-ring substance) selected the orange, while the nucleoli, 

 sperm-granules, centrosomes, and the large and small granules 

 in the spindle, attraction spheres, and cytoplasm selected the 

 methyl green. I did not suspect that many of these granules 

 might be deutoplasmic, for at that time I was fully convinced 

 that xylol or xylol balsam would, in all cases, dissolve out the 

 deutoplasmic granules in a few hours. Further investigation 

 showed me that the time required to dissolve the deutoplasmic 

 granules is very inconstant. In some cases it will require 

 days, again it will require as many weeks, and in a few 

 cases they form an insoluble compound with the stain, and 

 cannot be dissolved at all — this last being true even when the 

 sections have been subjected to exactly the same technique, in 

 the one case the deutoplasmic granules staining weakly and 

 readily dissolving out, and in the other staining deeply and 

 remaining insoluble. These facts led me to suspect their 

 identity with many of the above-mentioned methyl-green gran- 

 ules, and I am greatly indebted to Miss Strobell for assisting 

 me in my experiments to determine this point. 



Katharine Foot. 



To test the surmise that some of the granules differentiated 

 with methyl green were identical with the deutoplasmic gran- 

 ules, a number of eggs were submitted to the following technical 

 tests. The eggs were killed in chromo-acetic, as this fixative 



