No. 3-] THE EGG OF ALLOLOBOPHORA FOETID A. 133 



with the stain and cannot be dissolved out. Photo. 9 is a section 

 of an ovarian Q^'g, in which these granules stained intensely with 

 iron haematoxylin, all the sections in this slide giving the same 

 reaction. The next slide of sections of the same ovary was 

 treated similarly, and the osmophile granules did not stain. 



In a few cases we have completed the entire process of 

 killing, sectioning, staining, and mounting, within ten hours ; 

 and in these cases the granules have responded sharply to the 

 stain, but we have not repeated this method often enough to 

 test its value. 



Forviatioji of Vesicles between First and Second Maturation 

 Spindles. — In earlier papers (4-6) one of us has stated that at 

 the telophase of both the first and second maturation spindles, 

 the chromosomes assume the form of small vesicles, corre- 

 sponding in number to the number of the chromosomes. 



Mead (8) has seen in Chaetopterns similar vesicles at the 

 telophase of the second maturation spindle. He describes the 

 formation of these as follows : " When the chromosomes have 

 reached a position near the poles of the spindle, each of them 

 swells up to form a vesicle, in which, at first, two distinct rows 

 of granules may be seen. Later, each chromosome exactly 

 resembles a miniature nucleus." Whether the chromosomes 

 of the second maturation spindle of AllolobopJiora foetida form 

 their vesicles in this way, we are unable to state, as we have 

 not yet secured preparations of the 



second maturation spindle showing Q^ 'y - /-^ 



the metamorphosis of the chromo- /f ^ «*»^ I \ ^^. 

 somes to vesicles. Of the first '^ ^^ CJ \J 



maturation spindle, however, we ^^& /^ ><^ 



have preparations showing these 



, •.• 1 . 1,1 Fig. II. — Some forms shown by the chro- 



transitional stages, and they sug- „„3„„,, ^, „,, ^,i„ph,3, „,- .^^ ^^^^ 



geSt a method of formation differing maturation spindle, showmg the proba- 



ble method of formation of the eleven 

 from that of the second maturation vesicles, which are present a little later. 



spindle of Chaetopterns. Text-fig. c)!"photo^tx' ' """' '""""" °'' ''' 

 II represents a number of forms 



assumed by the daughter-chromosomes of the tetrads of the 

 first spindle. At the telophase of this spindle (Photo. 11) 

 we find these forms both in the polar body and Q.gg. They 



