134 



FOOT AND STROBELL. 



[Vol. II. 



suggest that the vesicles of this stage are formed by the two 

 parts of each chromosome uniting to form a ring. 



Living Cytoplasm. — The cytoplasm of the living egg of 

 Allolobophora foetida presents a dissimilar structure at different 

 stages of its development, and it has been our aim to demon- 

 strate this difference and 

 to determine, if possible, 

 what structures in the sec- 

 tions can be identified with 

 those seen in the living tgg. 



In an ^g^ at the pro- 

 nuclear and first cleavage 

 stages we have a definite 

 cytoplasmic feature, which 

 is not so pronounced in the 

 less mature ^g,%. This fea- 

 ture is conspicuously shown 

 in Photo. I in the form of 

 globules of varying sizes, 

 which appear to be an ap- 

 proximately transparent 

 non-miscible substance 



suggesting drops of sap or oil. We have designated them as 

 sap globules rather than oil globules, as they do not blacken 

 with osmic. 



In the living oocyte, first order, we have not been able to 

 demonstrate this structure (Text-fig. Ill), but in oocyte second 

 order we find very tiny sap globules (Text-fig. 

 IV) which gradually increase in size as the Qgg 

 matures (Photos. 4-5, I); (the globules develop- 

 ing whether the egg is fertilized or not). 



In abnormal eggs the sap globules are rela- 

 the early stages of a pathological 



Fig. III. — Living unfertilized egg, from perfectly 

 white cocoon. Only the larger osmophile granules 

 represented. Chromosomes distinctly visible. Cam- 

 era, 2 mm. immer., oc. 2. 





Fig. IV. — Peripheral 

 sap globules after for- 

 mation of ist polar tivelv laro"e 



body. Traced from ..... 



photographic nega- couditiou bcing apparently expressed by a too 

 tive of living egg. i-^pj^j development of the cytoplasm. The nor- 

 mal enlargement of the globules, as expressed 

 in later stages, cannot be due to individual growth, for the 

 increase in the size of the &gg is not at all commensurate with 



