Inorganic constituents. — On evaporation and ignition the fluid 
yielded a fair amount of light friable ash, almost insoluble in 
distilled water, though readily soluble in dilute hydrochloric acid. 
No quantitive estimation of ash was undertaken, as it would 
require more than was available to do so with any accuracy. When 
the secretion is allowed to stand overnight, it becomes turbid, 
owing to crystals visible to the naked eye, making their appear- 
ance in it. Under the microscope these crystals were seen to 
consist of colourless tufts, similar in appearance to those found in 
certain urines and termed « stellar phosphates », which is another 
term for calcium phosphate. The deposited crystals were carefully 
filtered off, washed, dissolved in dilute hydrochloric acid, and 
subjected to the usual analysis. On evaporation and incineration 
a white mass with no charring was obtained, in which the ions of 
calcium and phosphoric acid were easily revealed by the usual 
tests. Therefore the crystals were undoubtedly of calcium phos- 
phate. 
Search for the other inorganic constituents was then made, a 
quantity of 2 c.c. being sacrificed for this purpose. The organic 
matter was first removed by treatment with aqua regia and 
subsequent evaporation three times with hydrochloric acid. The 
solid thus obtained taken up in the smallest amount of dilute 
hydrochloric acid and subjected to the usual course of inorganic 
analysis with presence of phosphates. Owing to the considerable 
amount of calcium phosphate present the chief constituents were 
Ca" and PO;”. Somewhat considerable amounts of iron were, 
however, found, and also magnesium, potassium, and sodium. 
These various metals seemed to be chiefly present in the form of 
phosphates and chlorides, as no other acids could be detected. 
The proteins. — The fluid gives all the protein reactions very 
readily : thus alcohol, mercuric chloride, picric acid, nitric acid and 
other protein reagents give very bulky and solid precipitates. The 
Xanthoproteic and Millon’s reaction are both obtained, as is also 
Piotrowsky’s reaction, but the colour is masked by the pigmented 
nature of the liquid itself. Heat coagulates the proteins, the greater 
part being thrown down at 75-80° C.; the coagulum thus formed 
carries down most of the pigment with it. 
When the secretion is added to several times its bulk of water, 
a bulky light precipitate is obtained. When this precipitate is 
