Dev Or Eo ON sive Chi LAT Rh PRO TOZ OA 
OF (EAH O RE. 
By B. l,. Buatia, M.Sc., Assistant Professor of Zoology, 
Government College, Lahore. 
I. ON THE OCCURRENCE OF THREE CONTRACTILE VACUOLES IN 
SPECIMENS OF PARAMAECIUM CAUDATUM. 
Paramaecium caudatum, Ehrbg., is found in large numbers 
both in infusions and in stagnant water in Lahore and is studied 
as a type by the students. Recently (June, 1916) when my B.Sc. 
class were examining this animal, my attention was drawn by two 
students, Mr. Sham Nath and Mr. Prashar, to the existence of a 
third contractile vacuole in the specimen which was being studied 
by each. None of the other specimens from the same tube showed 
this peculiarity. The water had been collected from a ditch outside 
the laboratory compound. 
In both these specimens the two normal vacuoles, each with 
its own system of radiating canals, were situated at about one- 
fourth of the length of the animal from either end, as is usually 
the case. The third contractile vacuole was situated nearer to the 
posterior vacuole at one-fourth of the distance between the two 
vacuoles in one specimen, and midway between the two vacuoles 
in the other. In both specimens the third vacuole had its own 
system of radiating canals and showed its systole and diastole 
independently of the other two, the three contracting regularly 
one after the other. 
So far as I am aware, this occurrence of a third contractile 
vacuole has not been observed in this species before though it was 
observed by Butschliin P. putrinum, as recorded in the following 
extract from Bronn’s Thier-Reichs (1, p. 1417) :— 
‘‘Erhohung der vacuolenzahl ist nur von Paramaecium und 
Ophryoglena bekannt. Bei ersterer Gattung scheint die Zweizahl 
Regel zu sein (3 beobachtete Biitschli zuweilen bei P. purtrinum 
1876, p. 88); beide Vacuolen liegen ungefahr auf den Grenzen des 
I und 2 sowie des 3 und 4 KOrperviertel hintereinander.”’ 
II. RECORDS AND DESCRIPTIONS OF SPECIES. 
The object of this paper is to record and describe a number 
of the more striking Ciliata that the writer has come across during 
the last two years in the water collected at various times from 
ditches, ponds, etc., in and about Lahore. The list represents 
