IV. STUDIES ON INFUSORIA.— II. 

 On two new species of Holophrya, Ehrbg. 



By Ekendranath Ghosh, M.Sc, M.D. 



The genus Holophrya, Elirbg. may be briefly diagnosed as 

 follows : — Infusoria with cylindrical to spherical body entirely and 

 uniformly covered with cilia arranged in close meridional rows. 

 Cytostome antero- terminal or rarely slightly lateral, rounded or 

 rarely slit-like. C3^topharynx generally present and without rod- 

 apparatus, absent in a few species. Macronucleus one, sometimes 

 two or numerous macronuclei. C.V. one or more, then either 

 scattered or arranged in longitudinal rows. Division by transverse 

 fission. Free-swimming, freshwater and marine. 



The above diagnosis does not include the species H. muUi- 

 filiis considered by Schewiakoff (5) to belong to this genus. Being 

 parasitic and having a complicated life-history, it is left in a genus 

 of its own under the name I chthyophlhirius muUifiUis, Fouquet. 



Holophrya bengalensis, sp. nov. 



-Cylindrical with rounded 



The species may be diagnosed thus 

 ends, slightly stouter posteriorly ; the 

 cilia are long. Cytostome small and 

 circular, at anterior end; cytopharynx 

 absent ; macronucleus broadly fusiform 

 and placed in the middle of the body 

 near one side ; C.V. single, subterminal, 

 placed close to one side. I^ength 0*075 

 mm. ; breadth 0"037 mm. 



A single specimen was found in vege- 

 table infusion. 



Holophrya annandalei, sp. nov. 



The species may be diagnosed as 

 follows : — Cylindrical, rounded at both 

 ends, three times longer than broad; 

 cytostome antero-terminal and circular fjg. i, 

 in outline; cytopharynx a slight de- 

 pression; ciliary striae faint; macro- 

 nuclei two in number and spherical in shape, one placed in the 

 middle on one side and the other towards the anterior end. 

 C.V. single, placed at the junction of the anterior and middle third 

 of the body on one side. I^ength 0"i5 to 0*22 mm. 



-Holophrvn beng(ile)i- 

 sis, sp. nov. 



