Ig10.] J. STEPHENSON: Aquatic Oligocheta of the Punjab. 69 
that author, buds at all times except when in an advanced condition 
of sexual development: In the present species however the limita- 
tion just expressed does not hold; all the specimens I have seen, 
whether sexually ripe or not, show one or more zones of budding ; 
the single individual is never met with, but always a chain. 
The testes seem to disappear early, and I could not distinguish 
them in a specimen which showed sperm-morule in all stages of 
development, but no other genital organs, male or female, except 
the ovaries in an early stage. A small granular mass situated 
near the ventral body-wall and a short distance in front of the fun- 
nel of the vas deferens, seen in a specimen (plate viii, fig. 4) which 
had developed the male efferent apparatus but not the clitellum, 
perhaps represented a testis, whose disappearance may have been 
somewhat delayed. 
The funnel of the vas deferens is in segment v, on the anterior 
face of septum 5/6; it is ciliated. From it the vas deferens runs 
backwards in segment vi, with a somewhat curved course in living 
specimens, to the atrium; this is an oval or stoutly spindle-shaped 
dilatation of the tube, from which a short ejaculatory duct leads 
to the exterior. The male aperture is at the level of the sete of 
segment vi, which are modified as described below (genital sete). 
There is no prostate. 
There is also no sperm-sac. Sperm-morule in all stages of 
development are scattered throughout the body-cavity of the 
animal; and not only through the body of the anterior animal 
which contains the reproductive organs, but through all the mem- 
bers of the chain. Spermatozoa may be seen passing from one 
segment to another through the incomplete septa, and may reach 
as far forwards as the pharyngeal region. 
The ovaries are two cellular masses (described as testes in my 
former paper) in segment vi, at the level of or slightly anterior to 
the atrium on each side. They seem to appear early ; one speci- 
men showed two hyaline protoplasmic aggregates in which cell 
outlines were not (in the living condition) visible; these were sus- 
pended in the ventral part of the body-cavity on fine strands pass- 
ing between the alimentary canal and body-wall, one on each side 
at a level a little in front of the setze of segment vi; with the excep- 
tion of sperm-morulz, these, which were apparently an early stage 
of the ovaries, were the only signs of sexual organs. 
There is no ovisac, and the ova ripen in the general body- 
cavity. Though ova are sometimes ‘met with in the posterior 
animals of a chain, they are more usually found collected in the 
posterior part of segment vi, and here may cause a fairly definite 
backward bulging of the septum. The larger size of the egg-masses 
as compared with the sperm-morule probably occasions the restric- 
tion of their wanderings. What I figured inmy earlier paper as an 
ovary is such a collection of ova. 
The ripe eggs are of considerable size, and consist mainly, like 
those of the species of Nats previously described, of aggregations 
of glancing particles of yolk, opaque in mass by transmitted light. 
