100 APPENDIX. 
action upon rabbits, Cats were very susceptible to the poison, 
death following three days after the subcutaneous injection of 0-001 
gram. In these animals, 2 to 3 hours after the injection violent and 
repeated vomitings occurred, at first of food, and afterwards of mucus 
tinged with bile, and containing some intestinal worms ; there was 
also obstinate diarrhea. Finally the animals became dull, the 
temperature fell, the limbs were paralysed, and death occurred 
from exhaustion. Dogs, pigeons, and fowls were similarly 
affected. 
The post-mortem appearances observed were intense irritation, and 
sometimes abscess at the seat of the injection, the stomach normal or 
slightly injected, the upper portion of the mucous membrane of the 
duodenum highly injected, especially round the opening of biliary 
duct, the lower portion covered by a mass of brown epithelium 
mixed with detritus and mucus ; the lower part of the small intestine, 
and the whole of the large intestine, was covered by hemorrhagic 
patches, and here and there by a membranous exudation, with intense 
inflammation of the adenoid tissue, the sub-mucous and muscular 
layers not being affected. Liver congested, gall-bladder swollen 
and full of bile. Kidneys congested, with marked glomerulo- 
nephritis and commencing tubular nephritis, The distended . 
bladder and injection of the duct appearing to indicate elimination 
of the drug through the liver, the author tied the latter in three of 
the dogs experimented upon, and afterwards injected podophyllotoxin 
beneath the skin ; the results were exactly the same-as in the case of 
the dogs not previously so treated, 
Injections of podophyllotoxin into the veins gave exactly the same 
results as when it was administered internally or injected sub- 
cutaneously, The circulation, respiration, and nervous system were 
only affected a little before the fatal termination in all the animals 
experimented upon. 
From these experiments the author concludes that the drug acts 
as a simple irritant, and that its purgative action when given in- 
ternally is due to irritation of the intestinal canal. When injected 
under the skin or into a vein, it is eliminated by the blood through 
the kidneys and intestine, and in its passage through these organs it 
sets up the irritative action already described. 
