184 APPENDIX. 
In addition to the seed tinctures, we also prepared, for the purpose 
of comparison, three series of tinctures from the leaves, menstrua of 
the same alcoholic strength, and the same proportion of drug to 
smenstruum being used, as in the case of the seed tinctures, 
Before proceeding to the quantitative estimation of the alkaloid, a 
few preliminary experiments were tried, in order to ascertain whether 
the process employed for the estimation of the alkaloid in tinctures 
of henbane and belladonna was equally well adapted for the 
estimation of the stramonium tinctures, 
For this purpose 300 ¢.c. of a standard tincture was prepared 
with a 60 per cent, menstruum, and the alkaloid estimated by the 
following processes, the usual precautions being taken to prevent 
loss of alkaloid in washing with chloroform, &c.:— 
Experiment I.—Fifty c.c. of the tincture was evaporated to low 
bulk with addition of water, until all spirit had been removed, The 
residual liquor was allowed to cool and was then acidified with 
dilute sulphuric acid, and freed from fat and colouring matter b 
means of chloroform. It was then made alkaline, and the alkaloids 
removed by shaking with three successive portions of chloroform. 
From the mixed chloroformic solutions the alkaloids were extracted 
by three agitations with acidulated water, and were afterwards 
regenerated from the mixed acid solutions, after addition of excess 
of ammonia, by shaking out with chloroform, The latter solution 
was then shaken with ammoniated water, and after separation was 
drawn off and evaporated, and the residue dried at 100° and 
weighed. 
Experiment IT,—The tincture was evaporated to low bulk, the 
residual liquor allowed to cool, and an excess of dilute sulphurie 
acid added. It was then freed from fat and colouring matter by 
means of chloroform, a slight excess of ammonia added, the alkaloids 
shaken out with three doses of chloroform, the latter solutions 
mixed, and after treatment with ammoniated water evaporated, and 
_the residue dried and weighed, 
Experiment III.—The tincture was evaporated till all spirit was 
removed, and the residual liquor acidified with dilute sulphuric acid 
and shaken with chloroform and ether in turn, till the latter. came 
away colourless. The alkaloidal solution was then made alkaline 
with ammonia, the alkaloids shaken out with three successive 15 C.€, 
