APPENDIX. 185 
ether (sp. gr. *717), the mixed ethereal solutions evaporated, and 
the residue dried and weighed. 
Fixperiment IV.—The tincture was evaporated to remove the 
spirit, the residual liquor acidified, and colouring matter removed b 
means of chloroform. It was then made alkaline and the alkaloids 
extracted with chloroform. From the chloroformic solution the 
alkaloids were removed by agitation with acidulated water, the 
latter solution treated with a slight excess of ammonia ; the alkaline 
liquid shaken with three successive portions of ether (sp. gr. +71 7), 
and the mixed ethereal solutions evaporated, and the residue dried 
at 100°, and weighe 
Ezperiment V. “This was conducted exactly as Ne: II., the oil 
being removed by means of petroleum ether before the jireligabiiity 
treatment with chloroform. 
The results were as follows :— 
Experiment I. 50c.c. tincture = ‘014 gram alkaloids, 
015 
Experment II. 50cc.. , =° e 
Experiment ITI. 5@c.c. _,, = 12 m 
Experiment IV. 50 c.c. is = °010 ae 
Experiment V.50cc. , = 014 ee 
These results indicated that the method of extraction by means of 
chloroform was thoroughly reliable, notwithstanding the presence of 
fixed oil in the tincture, and also showed that the preliminary 
treatment with petroleum ether, in order to remove the oily matter, 
was unnecessary. Confirmatory experiments with a 70 per cent. 
tincture, by processes II. and V., gave the following results :— 
No. II. 50 ¢.c, tincture = 015 gram alkaloids, 
Moi Ne Ste. iy. ) = 014 is 
The exact details of the process adopted are as follows :— 
Fifty ¢.c, of the tincture to be estimated is introduced into a por- 
celain dish, and evaporated over a water-bath to low bulk; water 
being added, if necessary, until all the spirit is remove d, The resi- 
dual liquor is is allowed to cool, and is then acidified by the addition 
cotton wool into a separating funnel, The dish and filter are rinsed — 
first with a little acidulated water and then with 15 ¢.c, chloroform, 
the rinsings added to the contents of the funnel and the Bs) a : 
