lO ROYAL SOCIETY OF CANADA 



Two guiuea-pigs and two rabbits died spontaneously before tbe con- 

 clusion of the experiment, apparently from some gastro-intestinal dis- 

 turbance. The rest of the pigs were killed after thirty days, and one- 

 half of the remaining rabbits about the same time. The first animals 

 killed presented so little pathological change that it was thought advis- 

 able to keep the remaining six for two weeks longer in the hope that the 

 lesions would be more marked. Autopsies were performed at various 

 times with the special object of determining the extent of the dis- 

 semination of the tuberculous virus, the effect of the serum injections, 

 if any, and the character of the bacilli of tuberculosis found in the 

 various parts. Portions of the organs were examined microscopically, 

 both by the hsematoxylin method and the modified Ziehl-Nielsen 

 method for tubercle bacilli. Smears were made from the organs and 

 stained for bacteria. Cultures were also taken from the organs. 



Abstracts of the autopsy protocols are herewith presented: — 



Guinea-pig I. 



Inoculated March 13th with 1 c.cm. of standard dilution of B. Tuberculosis 

 intraperitoneally. Killed April 15th. Abdominal wall at site of inoculation 

 red and inflamed, containing a small caseous mass. Three inguinal glands 

 on the left side enlarged, a smear from which gave tubercle bacilli. Spleen: 

 large, soft and pale. Other organs normal. Microscopical examination showed 

 the organs to be congested, but without obvious tuberculosis. The inguinal 

 glands showed inflammatory hyperplasia. Cultures from the heart-blood, bile 

 and liver were sterile after thirty days. 



■Guinea-pig II. 



Inoculated intraperitoneally. Died on twenty-fifth day. One or two 

 small nodules, not caseous, in the abdominal wall at the site of inoculation. 

 One minute elevated spot of yellowish colour on right testis and four or 

 five similar ones on the left, surrounded by an inflammatory areola. Micro- 

 Bcopically, these were composed of granulation tissue, small round-cells, 

 and fibroblasts. Tubercle bacilli were demonstrated. Other organs normal. 

 Cultures from heart-blood and spleen, sterile after thirty-four day»- 



GUINEA-PIG III. 



Killed April 15th. Inoculated in left leg. Slight haemorrhage in region 

 of the inoculation wound. Two or three glands in the left inguinal region 

 enlarged, soft, and caseous looking. Smears from these gave the B. Tuber- 

 culosis. Other organs normal. Cultures from liver, bile, and heart-blood 

 sterile. Cultures from the affected glands and from the liver did not develop 

 tubercle bacilli. 



GUINEA-PIG IV. 



Killed April 15th. Inoculated in peritoneal cavity. Given goat serum, 

 2 c.cm. every second day. Anterior abdominal wall intensely haemorrhagic 

 from the inoculations. Two or three inguinal glands on the left side, and 

 a few mesenteric glands enlarge but films did not show tubercle bacilli. All 

 organs normal. Cultures from the heart-blood, liver, and bile, sterile. 



