[NiCHOLLs] BLOOD SERA IN TUBERCULOUS INFECTION 15 



Spleen much enlarged and almost full of confluent caseous tubercles. Liver 

 full of miliary tubercles. Other organs free. Smears from the spleen, liver, 

 and anterior mediastinal glands gave B. Tuberculosis. Cultures from heart- 

 blood and spleen, sterile. 



Microscopic examination. Heart: cloudy. Lung: Slight acute bronchitis, 

 collapse and haemorrhagic infarction, slight leucocytic infiltration in septa. 

 tipleen: Large coalescing tubercles with central necrosis, and chromatolysis. 

 Cells chiefly lymphocytes. No attempt at flbrosis. Liver: Numerous large 

 miliary foci, of which the largest showed slight caseation. The tubercles 

 were in the portal sheaths, and were composed chiefly of lymphocytes 

 together with fairly numerous epithelioid cells staining well. No fibrosis. 

 The remaining liver cells cloudy and fatty. Kidney: Cloudy. Lymph-gland (1): 

 Large caseous foci with chromatolysis, very few epithelioid cells and no 

 fibrosis. Lymph-gland (2): Similar, but with slight proliferation of fibrous 

 tissue at the periphery of the caseous foci. Abdominal wall: Section showed 

 the muscle to be cloudy and in parts infiltrated with lymphocytes and numer- 

 ous epithilioid cells. No caseation nor fibrosis. 



Guinea-pig IV. 



Inoculated intraperitoneally. On twenty-first day some induration of 

 the abdominal wall was observed and in two days more a small pustule 

 formed. In three days this opened spontaneously, and smears gave moderate 

 numbers of tubercle bacilli. Killed July 30th. 



At site of inoculation were numerous pearly and caseous masses in the 

 abdominal wall and over the peritoneal membrane generally. Great omentum 

 converted into a thickened caseous mass. Spleen and liver much enlarged 

 and riddled with miliary tubercles. The diaphragm presented numerous 

 pearly tubercles. A few retroperitoneal and numerous mesenteric glands 

 enlarged and caseous. Lungs showed miliary tubercles; the anterior medias- 

 tinal glands enlarged and caseous. Kidneys, cloudy. Smears from the 

 mesenteric glands and peritoneal fluid, negative. 



Microscopic examination. Heart: Cloudy. Lung: Fairly numerous tuber- 

 cles in the perivascular lymph spaces, consisting of lymphocytes and a few 

 leucocytes. No caseation nor fibrosis. Parts of the lung showed congestion 

 and alveolar catarrh. ISpteen: Great hyperplasia and large caseous masses. 

 Slight overgrowth of stroma. One giant-cell seen. Liver: Numerous and 

 rather large early tubercles in the portal sheaths consisting mainly of 

 lymphocytes. A few giant-cells seen, but no fibrosis. Lymph-gland: mark- 

 edly caseous, but without fibrosis. 



An interesting feature in this animal was that it aborted about one 

 month before death and the uterus was enormously enlarged and caseous, 

 while caseous matter had been discharged per vaginam. 



Guinea-pig II. 



Inoculated intraperitoneally. In twenty-one days a very small lump 

 formed at the site of the inoculation. Died on the twenty-fourth day. 



A small caseous dot found in abdominal wall at the site of inoculation. 

 Hsemorrhagic peritonitis. Clear fiuid found in both pleural sacs. The dia- 

 phragm showed no tubercles. Spleen very large, hsemorrhagic, and filled 

 with caseous masses. Liver much enlarged and filled with what appeared 

 to be caseous infarcts. Right lung contained a small caseous point sur- 



