[nichollsJ blood SERA IN TUBERCULOUS INFECTION 19 



rather numerous epithelioid cells, lymphocytes and polymorphonuclears. One 

 or two giant-cells present. Mesenteric gland showed simple hyperplasia. 



Rabbit V. 



Inoculated in leg. Given serum. On twelfth day two glands enlarged 

 in left inguinal region and one on the right. Killed on fifty-fifth day. A 

 collection of tubercles found on the fascia of the left leg at site of inoculation, 

 extending up the leg along the lymphatics. Rather dry. One retroperitonea', 

 and two mesenteric glands enlarged. 



Microscopical examination. All organs normal, or at most cloudy. Cul- 

 tures from blood, sterile. 



Rabbit VIII. 



Inoculated intraperitoneally. On twenty-first day slight induration at 

 site of inoculation. Killed on the fifty-first day. Infiltration the size of 

 a pea at the site of inoculation which gave B. Tuberculosis. The psoas 

 muscle on the right side inflamed, reddened, and on cutting into it it appeared 

 caseous. Numerous pearly miliary tubercles scattered over peritoneum, 

 mesentery, and omentum. Diaphragm showed a few miliary tubercles. A 

 few retroperitoneal glands enlarged and caseous. A few rather large tuber- 

 cles in the lower lobe of the right lung; one or two in right. Possibly a 

 few minute tubercles in the liver. Spleen enlarged, but not visibly tuber- 

 culous. One or two mesenteric glands enlarged. A smear from the abdom- 

 inal wall and also from the spleen gave the B. Tuberculosis. Culture from 

 blood, sterile; from retroperitoneal gland, staphylococcus. 



Microscopical examination. Lung: one or two small miliary foci com- 

 posed of lymphocytes and epithelioid cells, without caseation. Small round- 

 celled infiltration in the septa. Spleen: Congested, hyperplasia of follicles. 

 Liver: A few minute collections of lymphoid cells with slight fatty degenera- 

 tion. Section of Wound: Chronic granulation, with fragmentation and swelling 

 of the muscle fibres. 



Rabbit X. 



Inoculated intraperitoneally. Given serum. On twenty-first day slight 

 induration at the site of inoculation. Died on forty-seventh day. Large 

 subcutaneous abscess in right fiank caused by the inoculations. All organs 

 found normal, except for congestion or cloudiness. 



It was found in the course of this experiment that after the injec- 

 tion of the bacilli the average temperature of the animals was raised one 

 degree. The average temperature of all the animals, both pigs and 

 rabbits, was 102.52° before inoculation; after, it was 103.41° in those 

 animals not receiving scrum and 103.62 in those given it. In the case 

 of the control animals that were given serum alone (Nos. "VT and IX.), 

 the temperature in one was only slightly elevated, in the other normal. 

 We may thus conclude that the injection of the serum had no effect on 

 the temperature curve. With regard to the weights the result was dif- 

 ferent. The animals receiving serum lost 22.27% of their body weight; 

 those not receiving it lost only 10.45^. As a rule rabbits inoculated 



