[nicholls] blood sera IN TUBERCULOUS INFECTION 23 



tion of the tubercle germs the average temperature was 103.7° and the 

 weight 1,878 grms. The average temperature before inoculation with 

 tuberculosis of the rabbits that did receive serum was 103.3° and the 

 average weight was 1360 grms. ; after inoculation with tuberculosis and 

 after receiving antitoxic serum the average temperature was 103.3° and 

 the weight 1673. Thus, as the culture inoculated was innocuous, the 

 conclusion is that the antitoxic serum had no effect on the temperature 

 while it apparently stimulated nutrition as the animals receiving it had 

 markedly increased in weight, and in truth appeared in fine condition. 

 This latter result, so different from that of the first two experiments, is 

 explained from the fact that by a modification of the method the injec- 

 tions produced but little local disturbance, and the amount of irritation 

 was reduced to a minimum. Finally as the experiment had to be con- 

 cluded rather hastily, six guinea-pigs were taken, their normal tem- 

 perature ascertained, and they were grouped in pairs as before accord- 

 ing to weight. Two were inoculated in the left leg with a standardized 

 emulsion of relatively mild bacilli (one c. cm.), and the remaining four 

 intraperitoneally with the same amount. One member of each pair was 

 given one c. cm. of antitoxic serum subcutaneously every second day. 

 Numbers III. and VI., inoculated in the leg, died on the second day of 

 the experiment, and presented no evidences of tuberculosis. Number 

 IV. 'died on the ninth day and its mate was killed on the eleventh. 

 Numbers I. and V. were killed on the fourteenth day. 



Autopsy protocols: — 



Guinea-pig I. 



No signs of tuberculosis at the site of inoculation in the abdominal wall. 

 No peritonitis. Two retroperitoneal glands on the left side were slightly 

 enlarged but not caseous. Films from these did not show the bacillus tuber- 

 culosis. ISpleen: Slightly enlarged and containing fair numbers of small 

 greyish dots resembling early tubercles; films from the spleen, however, did 

 not reveal the specific bacillus. A few small pearly nodules of greyish 

 colour seen in the great omentum. A small yellow streak was seen in the 

 liver not unlike a tubercle, but a smear from this did not show the tubercle 

 bacillus. 



Microscopical examination. Great omentum: The greyish dots referred 

 to proved to be early tubercles. Lung: Slight bronchitis and peribronchitis. 

 Liver: Cloudy. Spleen: Marked hyperplasia of the Malpighian bodies; no 

 obvious tuberculosis. This animal had been given serum. 



Guinea-pig V. 



Mate of the last, receiving no serum. No signs of tuberculosis at site of 

 inoculation in the abdominal wall. Two small retroperitoneal glands were in 

 evidence, not caseous. One tubercle on right testis. A few mesenteric 

 glands were slightly enlarged. Spleen much enlarged and full of whitish-grrey 



