24 ROYAL SOCIETY OF CANADA 



dots the size of pin-heads resembling tubercles. Smears from the spleen gave 

 tubercle bacilli. 



Three isolated tubercles the size of hemp-seeds noticed in the liver. Films 

 from these showed the specific bacillus. The great omentum was notably- 

 thickened, being converted into a transverse cord of firm gelatinous appear- 

 ance. The pleural cavities contained a fair quantity of rather blood stained 

 fluid. In this case the amount of tuberculosis was greatly in excess of that 

 in the last animal. 



Guinea-pig II. 



Given serum, igriight redness and oedema at the site of the injection of 

 the serum. No signs of tuberculosis at the site of inoculation in the abdom- 

 inal wall. Two retroperitoneal glands near the pelvis swollen, but not 

 caseous; films gave a few tubercle bacilli. Some minute pin-point nodules 

 of pearly grey appearance were found in the omentum. Two larger nodules 

 also found in the omentum, one of which was caseous. Other organs normal. 



Microscopical examination. Abdominal toall: Considerable extravasation 

 of leucocytes about the blood-vessels. Infiltration between the muscle fibres 

 of leucocytes and considerable numbers of epithelioid cells. Muscle-fibres 

 cloudy. No caseation. Retroperitoneal gland: Hyperplastic, but not caseous. 

 Liver: Fatty, one or two minute areas composed of, in the main, clear 

 mononuclear cells, resembling epithelioid -cells. Spleen: Much congested with 

 hyperplasia of the Malpighian follicles; no caseation. Kidney: Normal. The 

 nodules in the great omentum proved to be early tubercles. 



Guinea-pig IV. 



Two elands enlarged but not caseous under the skin near the site of the 

 Inoculation, with congestion of the vessels in the neighbourhood. One retro- 

 peritoneal gland on the left side near the pelvis considerably enlarged and 

 oaseous. A film from this gave the B. Tuberculosis. A few mesenteric 

 glands near the cœcum enlarged but not caseous. In thé great omentum were 

 numerous small gelatinous tubercles and caseous foci. Other organs free. 



Microscopical examination. Liver: Congested and cloudy. Kidney: Cloudy. 

 Lung: A few small well defined rounded areas about the vessels composed 

 mainly of cells of epithelioid type, but with a few leucocytes; no caseation. 

 Spleen: Considerable blood-pigment noticed. Hyperplasia of the Malpighian 

 follicles. Retroperitoneal gland: Fairly extensive central caseation. This 

 animal had not been given serum. Comparing this animal with its mate 

 (No. IL), the amount of tuberculosis was notably greater, and this was even 

 more marked when we take into account that the duration of the disease 

 was two days less. 



While this experiment was in progress the two rabbits that had 

 previously been inoculated in the anterior chamber of the eye were kept 

 under observation. One apparently had received some secondary 

 infection at the operation as the eye was very rapidly destroyed 

 and no safe conclusions could be drawn from it. The other proved 

 more satisfactory hoAvever. For about two weeks the small caseous' 

 masEi in the anterior chamber resulting from the injection slowly 

 enlarged to about twice its original size. With this there was' consider- 



