56 



THE ROYAL SOCIETY OF CANADA 



Two frogs were removed alive and allowed to remain in air, in 

 vessels the bottoms of which were kept covered with water. Sub- 

 sequent observations on them are of some interest. The first, a female, 

 was removed after 4| days, badly swollen, and buoyant. It com- 

 menced to breathe at once, and, as will be seen in the following table, 

 rapidly lost both liquid and gas, the former somewhat more quickly. 

 The second frog, also a female, after 15 days immersion was apparently 

 dead at the bottom of the containing vessel, and tapping elicited no 

 response. It was very swollen, but not lighter than water (neither 

 rising nor sinking). On removal it at once commenced to breathe, 

 and, as the table shows, rapidly lost absorbed water. 



TABLE IL 



The slight irregularities are largely due to the difference in volume 

 found when the frogs were immersed during expiration or during 

 inspiration. The increase in weight and volume which took place 

 in the first frog during the fourth to ninth days of observation was 

 probably due to the fact that the containing vessel contained so much 

 water that a considerable area of the frog's surface was immersed, 

 and the water intake was greater than the output. 



At the end of the fourteenth day of observation the first frog, 

 which had steadily become more active as it diminished in size and 

 volume (lethargy is one of the noticeable accompaniments of the 

 swelling), and which was now apparently quite normal, was pithed 

 and examined. The lateral lymph spaces and abdomen contained 

 only a drop or two of serum. 



