[cameron-brownlee] gas in TISSUES OF THE FROG 57 



The second frog, which similarly had become very active, was 

 also pithed and examined at the end of the observation period, with 

 precisely similar results. 



Experiment 4. Frog No. 1 in Table I contained 4 c.c. of liquid 

 in the lateral lymph spaces, 3 c.c. in those of the hind limbs, and about 

 0-5 c.c. in the abdomen. The liquid coagulated to a clear clot within 

 10 minutes of removal. 



Frog No. 2 in the table contained 8 c.c. of liquid in the lateral 

 lymph spaces, 2. c.c. in the lower limbs, and a drop or two in the 

 abdomen. 



Frog No. 10 in the table contained 15 c.c. of liquid in the lateral 

 lymph spaces, 1 c.c. in the lower limbs, and 2 c.c. in the abdomen. 

 Many of the muscles were very oedematous. This is true generally 

 of such swollen frogs. 



These figures show typically the distribution of the absorbed 

 liquid. 



Two frogs of this experiment were removed from water alive on 

 the thirteenth day (as already stated), with a view to ascertaining 

 the condition before death. Both were distinctly swollen and buoyant 

 before removal, and both breathed directly after removal. 



The first, a male, weighed 69-5 grams, and its volume was 76 c.c. 

 giving a density of 0-91, and gas absorption 6-5 c.c. Examination 

 under water, after pithing, showed the gas chiefly beneath the skin, 

 as usual. The residual weight was 54-5 grams, indicating that 15 c.c. 

 of liquid had been absorbed. 



The second frog, also a male, was very dropsical at each side 

 when removed. Its volume was 93 c.c, its weight 84-5 c.c, the density 

 being therefore 0-91 and the gas absorbed 8-5 c.c. The frog was 

 pithed under water, and cut open, and the gas removed very satis- 

 factorily and analysed. The residual weight was 65 -5 grams, indicat- 

 ing a liquid absorption of 19 c.c The gas removed measured (cor- 

 rected to normal pressure and 15°C.) 7-3 c.c, and contained an un- 

 measurable trace of oxygen, the pyrogallol solution being barely 

 coloured. 



These experiments, together with those in table II, show that 

 any changes after death can be neglected in considering previous 

 results, since the bulk of the changes observed take place during life. 



A sample of the skin from frog No. 6 in table I was hardened in 

 mercuric chloride, stained with haematoxylin, and examined micros- 

 copically with similar preparations from a normal frog. No marked 

 change was revealed. 



Experiment 5. The gases obtained by boiling Winnipeg tap- 

 water were analysed. They were collected over water, so that the 



