[HARRISON- ORGANISMS IN AESCULIN-BILESALT MEDIA 215 



vanderleck] 



Note 2. 



Sterile litmus milk is prepared as follows: — 



Twenty grams of blue litmus (Kahlbaum) are dissolved in 1,000 c.c. water 

 and sterilised. Of this solution enough is added to separated milk to give it 

 a distinct blue colour after the material is put in tubes. As a rule 40 c.c. litmus 

 solution to a litre of separated milk will be required. 



