[HARRISON] 



NITRO-CULTURES 



221 



Ashby's agar, wood ash agar and dipotassium phosphate agar 

 with the addition of various sugars were compared in the first series 

 of experiments. 



1% 1% 



Comm. C.P. 



Maltose Maltose 



Ashby's Agar. 

 Vetch 



Alsike 



Alfalfa 



Soy 



Red Clover. 



Wood Ash Agar 

 Vetch 



Alsike 



Alfalfa 



Red Clover. 

 Soy 



Di-Potass. Phosphate Agar 

 Vetch 



Alsike 



Alfalfa 



Soy. 



Red Clover 



xxxxx 



xxx 



xxxx 



xxxx 



XXX 

 XXX 

 XXX 



XX 

 XXX 



XX 





 



X 

 X 





 

 

 



XXX 



XX ! 





 



X 

 X 

 X 



XXX 

 XXX 



xxxx 



XXXX 



XXX 



XXX 



XXX 



X 



XX 



X 



XXX 

 X 



X 



XXX 



XX 





 



X 

 X 





 



XXX 

 X 



Mannite 



XX 



XX 



XXX 



XX 



XXX 



XXX 



X 



X 



X 



XX 



XXXXX 

 XXX 



xxxxx 



*xxxxx 



XX 



X 



XX 



XX 



*Best growth of series. 



Growth X 



Moderate Growth xx 



Spreading but thin xxx 



Thick but not spreading xxxx 



Thick and spreading xxxxx 



Bottom row of symbols — -7 days* growth. 

 Top row of symbols — 14 days' growth. 

 Room temp. 



Comparing all these cultures, the best growths as judged by myself 

 and independently by one of my assistants, Mr. W. Sadler, were — 

 the alsike culture on saccharose Ashby, the vetch culture on dextrine 

 dipotass, phosphate, and the alfalfa, soy and red clover on wood 

 ash agar. The vetch growth on wood ash agar was little inferior 

 to that on the dextrine agar. 



