[HARRISON] NITRO-CULTURES 223 



litres of medium are filled into ounce and a half wide mouth Blake 

 bottles, plugged with cotton. These bottles when filled are sterilised 

 in the autoclay at a pressure not exceeding 10 lbs. On removal, the 

 bottles are sloped, and inoculated by means of a pipette. About 2 

 c.c. of a suspension of the desired organism is run into each bottle. 

 The bottles are kept in a sloped position, and incubated at 25°C. for 

 about a week, when they are ready for distribution. 



References. 



Harrison & Barlow. Transactions of the Royal Society of Canada. Second series, 



1906-07. Vol. XII, Sect. 4, p. 157-237. 

 Harrison & Barlow. Co-operative experiments with Nodule forming bacteria. 



Bull. 148, Ont. Agr. College, Toronto, A'larch, 1906. 

 Edwards, S. F. & Barlow, B. Legume Bacteria. Bull. 169, Ontario Dept. of Agr., 



Toronto, 1909. 

 Lipman, J. G. Tests of Commercial Cultures for Soil Inoculation. Bull. 227. 



New Jersey Expt. Station, New Brunswick, 1910 (satisfactory returns from 



Farmogerm.) 

 Starnes, H. N. Some Field Notes on Soil Inoculation. Bull. 71, Georgia Expt. 



Station. Experiment Ga., 1905. 

 Harding, H. A . and Prucha, M. J. The Quality of Commercial Cultures for Legumes. 



Bull. 270, New York Expt. Station, Geneva, N.Y. 1905. 

 Grandeau, L. Journal Agr. Prat. N. Ser. 18. 1909. No. 45, page 625 and Nitro- 



bacterine. 

 Grabner, E. Journal Landw. 57 (1909) No. 3, page 217-223. 

 Feilitzen, H. Von. Centralblatt fur Backt. 2Abt. 26 (1910) p. 345-352. 

 Review of Reviews. Nitro-bacterine. Vol. XL, No. 238., Oct. 1909. 



