FAMILY IV. PSEUDOMONADACEAE 93 



dd. Attack cellulose 



e. Acid produced in milk. 



44. Pseudomonas tralncida. 

 ee. Milk unchanged. 



45. Pseudomonas lasia. 

 cc. Grow readily at 25° C. but poorly or not at all at 37°C. 



d. Action on cellulose unknown or unreported. 



e. Alkaline reaction in milk. Attacks riboflavin converting 

 it to lumichrome. 



46. Pseudomonas riboflavina. 

 ee. Reaction in milk unknown or unreported. 



f. Nitrates reduced to elemental nitrogen. 



47. Pseudomonas denitrificans . 



ff . Nitrates reduced to nitrites. Indole decomposed with 

 formation of blue crj'stals of indigotin. 



48. Pseudomonas indoloxidans. 

 dd. Attacks cellulose. 



49. Pseudomonas mira. 

 B. Sea-water and brine forms (a few are pathogenic). 



1. Produces pigments which are soluble in culture media. Gelatin liquefied. 



50. Pseudomonas nigrifaciens. 



2. Pigments soluble in culture media not produced. 



a. Gelatin liquefied. 



b. Polar flagellate. 



c. No growth in milk. 



d. "Indole produced; nitrites produced from nitrates. 



51. Pseudomonas ichthyodermis. 

 dd. Indole not produced; nitrites not produced from nitrates. 



e. Produces hydrogen sulfide and ammonia from tr5^ptone: 

 no acid from glucose. 



52. Pseudomonas marinoglutinosa . 

 ee. Hydrogen sulfide not produced; glucose acidified. 



53. Pseudomonas memhranoformis . 

 cc. Action in milk unknown or unreported. 



d. Digests agar. 



54. Pseudomonas gelatica. 

 dd. Do not digest agar. 



e. Deposit CaCOs in sea-water gelatin and in agar media in 

 old cultures. Do not grow in 12 to 30 per cent salt solu- 

 tions. 



55. Pseudomonas calcis. 



56. Pseudomonas calciprecipitans. 

 ee. Does not deposit CaCOs in sea-water gelatin or in agar 



media. Grows well in 12 to 30 per cent salt solutions. 

 56a. Pseudomonas halestorga. 

 bb. Non-motile. Cellulose attacked. Insoluble yellow pigment produced. 



57. Pseudomonas iridescens (gela- 



tin-liquefying variety), 

 aa. Gelatin not liquefied, 

 b. Polar flagellate. 



c. Does not attack cellulose. Produces an insoluble purjjle pigment 

 in vegetable extracts. 



58. Pseudomonas beijerinckii . 



