FAMILY IV. PSEUDOMONADACEAE 



101 



i^listening, yellowish gray to creamy. Dis- 

 agreeable odor. Medium becomes brownish 

 gray. 



Indole not produced. 



Nitrites not produced from nitrates. 



Acetylmethjdcarbinol not produced. 



Hydrogen sulfide not produced. 



Slightly acid, becoming alkaline in glu- 

 cose. No acid from arabinose, .xylose, lactose, 

 sucrose, maltose, trehalose, raffinose, man- 

 nitol, dulcitol, inositol or salicin. 



Starch not hydrolyzed. 



Pathogenic for guinea pigs and rabbits, 

 horned lizards, Gila monsters and chuck- 

 wallas. Marked hemolysis of rabbit cells 

 and slight hemolysis of Gila monster cells 

 suspended in agar. 



Temperature relations: Optimum, 20° to 

 25° C. Maximum, 37° C. A retest of several 

 strains of this organism by Haynes shows 

 that it grows well at 37° C. and is closelj^ 

 related to, though not identical with, 

 Pseudomonas aervginosa Migula. 



Distinctive characters: Yellowish green 

 fluorescence present only in meat infusion 

 media. The pigment is water-soluble, but 

 insoluble in chloroform. Pathogenic for 

 guinea pigs, rabbits, horned lizards and 

 chuckwallas. 



Source: Isolated from a bacterial disease 

 of horned lizards and Gila monsters. 



Habitat: Pathogenic for lizards. 



4. Pseudomonas caviae Scherago, 1936. 

 (Jour. Bact., 31, 1936, 83; also see Jour. Inf. 

 Dis., 60, 1937, 245.) 



ca'vi.ae. M.L. fem.noun Cavia generic 

 name of the guinea pig; from So. American 

 Indian, "cabiai", a guinea pig; caviae of 

 Cavia. 



Rods, 0.6 to 1.0 by 1.5 to 3.0 microns, oc- 

 curring singly and in pairs; rounded ends. 

 Motile by means of 1 to 3 polar flagella. 

 Encapsulated. Gram-negative. 



Gelatin stab: Infundibuliform liquefac- 

 tion. 



Agar colonies: Circular, convex, smooth, 

 iridescent and translucent, finely granular, 

 entire. 



Agar slant: Growth abundant, grayish 

 white, butyrous, filiform, glistening, trans- 

 lucent, markedly iridescent. Medium even- 



tually tinged greenish j'ellow, becoming 

 brownish yellow. 



Broth: Cloudy, pellicle, abundant light 

 yellow granular sediment, becoming brown. 

 IVIedium becomes j^ellow. 



Litmus milk: Acidified, coagulated, pep- 

 tonized, litmus partially reduced. 



Potato: Growth scant, filiform, glisten- 

 ing, light yellow to light orange, becoming 

 light brown. 



Hydrogen sulfide not produced. 



Indole produced. 



Nitrites produced from nitrates. 



Blood serum not liquefied. 



Blood not hemolyzed. 



Sodium formate decomposed. 



Catalase-negative. 



Methyl red positive; acetylmethylcar- 

 binol not produced. 



Citrate broth: No growth. 



Methylene blue reduced. 



Acid but no gas from glucose, fructose, 

 galactose, maltose, cellobiose, mannitol, 

 lactose, arabinose, sucrose, trehalose, sor- 

 bitol, mannose, dextrin, salicin, glycerol, 

 aesculin, am3^gdalin and starch. No acid 

 from xylose, dulcitol, rhamnose, inulin, 

 adonitol, raffinose, erythritol or inositol. 



Aerobic, facultative. 



Optimum temperature, 37° C. Grows at 

 25° C. 



Source: Isolated from guinea pigs dead 

 from epizootic septicemia. 



Habitat : From infected guinea pigs so far 

 as known. 



5. Pseudomonas boreopolis Gray and 

 Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 

 1928, 92.) 



bo.re.o'po.lis. Gr. boreas north; Gr. polis 

 a city; M.L. fern. gen. n. boreopolis of North 

 City. 



Rods, 0.5 to 1.0 by 2.0 to 3.0 microns, oc- 

 curring singly and in pairs. Motile with one 

 to five polar flagella. Gram-negative. 



Gelatin colonies: Liquefied. 



Gelatin stab: Liquefied. Medium red- 

 dened by some strains. 



Agar colonies: Circular or amoeboid, 

 white to buff, flat to convex, smooth, 

 glistening, translucent border. 



Agar slant: Filiform, whitish, raised, 

 smooth, glistening, fluorescent. 



