FAMILY IV. PSEUDOMONADACEAE 



105 



Indole not produced. 



Acid but no gas from glucose, fructose, 

 galactose and glycerol. No acid or gas from 

 salicin or raffinose. 



Aerobic. 



Grows well at 20° C. 



Distinctive character: Produces an in- 

 tense, diffusible, yellow to orange color in 

 cream or in the cream layer of milk. 



Source: Isolated from bitter milk. 



Habitat: Milk and cream so far as is 

 known. 



13. Pseudoinonas fluorescens INIigula, 

 1895. (Bacilhis fluorescens liquefadens 

 Fliigge, Die Mikroorganismen, 1886, 289; 

 Migula, in Engler and Prantl, Die natiirl. 

 Pflanzenfamilien, 1, la, 1895, 29.) 



flu.o.res'cens. L. fluor a flux; M.L. 

 fluoresco to fluoresce; fluor-spar, a fluxing 

 mineral which is fluorescent; M.L. part. 

 ad] . fluorescens fluorescing. 



Rods, 0.3 to 0.5 by 1.0 to 1.8 microns, oc- 

 curring singly and in pairs. Motile, posses- 

 sing a polar flagellum; occasionally non- 

 motile. Gram-negative. 



Gelatin colonies: Circular, with greenish 

 center, lobular, liquefying quickly; occa- 

 sionally viscid. 



Gelatin stab: Infundibuliform liquefac- 

 tion, with whitish to reddish gray sediment. 



Agar slant: Abundant, reddish layer, be- 

 coming reddish gray. The medium shows 

 greenish to olive-brown coloration. 



Broth: Turbid, flocculent, with yellowish 

 green pellicle and graj'ish sediment. 



Litmus milk: No coagulation; becoming 

 alkaline. 



Potato: Thick, grayish yellow, spreading, 

 becoming light sepia-brown in color; occa- 

 sionally viscid. 



Indole not produced. 



Nitrates reduced to nitrites and am- 

 monia. 



Acid from glucose. 



Blood serum liquefied. 



Aerobic. 



Optimum temperature, between 20° and 

 25° C. 



Not pathogenic. 



Source: Water, sewage, feces. 



Habitat: Soil, water and occasionally 



foodstuffs that have become contaminated 

 from these sources. 



14. Pseudomonas pavonacea Levine 

 and Soppeland, 1926. (Bull. No. 77, Iowa 

 State Agr. College, 1926, 41.) 



pa.vo.na'ce.a. L. adj. pavonaceus like a 

 peacock's tail, variegated. 



Rods, 0.5 by 4.5 microns, with truncate 

 ends, occurring singly and in chains. Old 

 cell'- develop 2 to 4 knob-like processes. 

 Sluggishly motile. Gram-negative. 



Gelatin stab: Crateriform liquefaction. 

 Medium becoming brown. 



Agar colonies: Circular, raised, becoming 

 green, amorphous, entire. 



Agar slant: Green, smooth, glistening, 

 viscid, medium becoming dark brown. 



Broth: Turbid, with viscid sediment. Me- 

 dium turned brown. 



Litmus milk: Slightly alkaline. Litmus 

 reduced. Peptonized after 10 days. 



Potato: No growth. 



Hydrogen sulfide produced. 



Indole not produced. 



Neither nitrites nor gas produced from 

 nitrates. 



Blood serum liquefied in 5 days. 



No acid or gas from glucose, lactose, 

 sucrose or glycerol. 



Aerobic, facultative. 



Optimum temperature, 22° C. Scant 

 growth at 37° C. 



Distinctive characters: Growth on solid 

 media distinctly green. Not fluorescent. 

 Medium becomes brown. 



Source: Isolated from activated sludge. 



15. Pseudomonas geniculata (Wright, 

 1895) Chester, 1901. (Bacillus geniculatus 

 Wright, Memoirs Nat. Acad. Sci., 7, 1895, 

 459; Chester, Man. Determ. Bact., 1901, 

 313.) 



ge.ni.cu.la'ta. L. adj. geniculatus jointed. 



Medium-sized rods, occurring singly, in 

 pairs and in chains. Motile, possessing 1 to 

 4 polar flagella. Gram-negative. 



Gelatin colonies: Circular, whitish, as- 

 sume a greenish shimmer, translucent. Deep 

 colonies yellowish. 



Gelatin stab: Infundibuliform liquefac 

 tion. Sediment light pink. 



