106 



ORDER I. PSEUDOMONADALES 



Agar slant: Grayish, glistening, translu- 

 cent, limited. Agar becomes brownish green. 



Broth: Turbid, with slight gray pellicle 

 and sediment. Broth becomes green. 



Litmus milk: Alkaline; reduction of lit- 

 mus; slight coagulation. Serum becomes 

 green. 



Potato: Thin, brownish, moist, glistening, 

 viscid. 



Indole not produced. 



Aerobic, facultative. 



Optimum temperature, between 20° and 

 25° C. No growth at 35° C. 



Source: From water from the Schuylkill 

 River. 



Habitat: Water. 



16. Pseudomonas septica Bergey et al., 

 1930. (Bacilhis fluorescens septicus Stutzer 

 and Wsorow, Cent. f. Bakt., II Abt., 71, 

 1927, 113; Bergey et al., Manual, 3rd ed., 

 1930, 169.) 



sep'ti.ca. Gr. adj. septicus putrefactive, 

 septic. 



Rods, 0.6 to 0.8 by 0.8 to 2.0 microns, oc- 

 curring singly. Motile with a polar flagel- 

 lum. Gram-negative. 



Gelatin stab: Infundibuliform liquefac- 

 tion. 



Agar colonies: Circular with opalescent 

 center and transparent periphery. 



Agar slant: Moderate, undulate margin. 



Broth: Turbid with fragile pellicle, green- 

 ish in upper portion. 



Litmus milk: Alkaline, coagulated. 



Blood serum not liquefied. 



Acid from glucose. 



Aerobic, facultative. 



Optimum temperature, 20° C. 



Source: Isolated from diseased caterpil- 

 lars. 



Habitat: From infected caterpillars so 

 far as known. 



17. Pseudomonas syncyanea (Ehren- 

 berg, 1840). Migula, 1895. (Vibrio syncyaneus 

 Ehrenberg, Berichte ii.d. Verh. d. k. Preuss. 

 Akad. d. Wissensch. z. Berlin, 5, 1840, 202; 

 Migula, in Engler and Prantl, Die natiirl. 

 Pflanzenfam., 1, la, 1895, 29.) 



syn.cy.a'ne.a. Gr. srjn- along w^ith, en- 

 tirely; Gr. cyaneus dark blue, dark; M.L. 

 adj. syncyaneus entirely blue. 



Rods with rounded ends, occurring singly, 

 occasionally in chains, 0.7 by 2.0 to 4.0 

 microns. Motile with two to four polar fla- 

 gella. Gram-negative. 



Gelatin colonies: Flat, bluish, translu- 

 cent. 



Gelatin stab: Surface growth shiny, 

 grayish blue. The medium is colored steel- 

 blue with greenish fluorescence. Gelatin is 

 liquefied. Some strains do not liquefy. 



Agar slant: Grayish white streak. The 

 medium takes on a bluish gray color with 

 slight fluorescence. 



Broth: Turbid with marked fluorescence. 



Litmus milk: Unchanged. In association 

 with lactic-acid bacteria the milk takes on a 

 deep blue color. 



Potato: Yellowish gray, shiny layer, be- 

 coming bluish gray. The tissue becomes 

 bluish gray. 



Indole not produced. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature, 25° C. 



Source: From milk that was bluish in 

 color. 



Habitat: The cause of blue milk. 



18. Pseudomonas iodinum (Davis, 

 1939) Tobie, 1939. (Chromobacierium io- 

 dinum Davis, Zent. f. Bakt., II Abt., 100, 

 1939, 273; Tobie, Bull. Assoc. Diplomes 

 Microbiol. Fac, Nancy, No. 18, 1939, 16.) 



i.o.di'num. M.L. neut.noun iodinum 

 iodine. 



Rods, 0.5 by 1.0 to 2.0 microns, occurring 

 singly. Non-motile. Gram-negative. 



Gelatin stab: Stratiform liquefaction. 

 Crystals of iodinin form. 



Agar colonies: Round, smooth, gray- 

 white, moist, glistening. Dark purple crys- 

 tals having the appearance of iodine crystals 

 form in the growth and in the adjacent 

 medium. This pigment is actually a phena- 

 zine di-N-oxide, there being no iodine 

 present (Clemo and Mcllwain, Jour. Chem. 

 Soc, Pt. 1, 1938, 479; Clemo and Daglish, 

 Jour. Chem. Soc, Pt. 1, 1950, 1481). 



Broth: Turbid. Crystals of iodinin form 

 on bottom of tube. 



Litmus milk: Alkaline; slow reduction of 

 litmus. 



