FAMILY IV. PSEUDOMONADACEAE 



107 



Potato: Viscous, creamy, spreading, be- 

 coming dark. 



Catalase-positive. 



Indole not produced. 



Nitrites produced from nitrates. 



No acid from carbohj-drates. 



Acetylmethylcarbinol not produced. 



Aerobic. 



Optimum temperature, 28° C. Grows at 

 37° C. 



Distinguishing character: The pigment, 

 iodinin, is readih' formed in any medium 

 containing soluble nitrogenous compounds. 

 Potassium and sodium citrates markedly 

 stimulate pigment production. Yeast ex- 

 tract is inhibitory to formation of iodinin, 

 which is soluble in benzene, toluene, xylene, 

 chloroform, carbon disulfide and ethyl ace- 

 tate. Such solutions are ruby red. The pig- 

 ment, like pyocyanin and chlororaphine, is a 

 phenazine derivative. 



Source: Isolated from milk. 



Habitat: Unknown. 



19. Pseudomonas smaragdina (Mez, 



1898) Migula, 1900. {Bacillus smaragdinus 

 foetidus Reiman, Inaug. Dissertation, 

 Wiirzburg, 1887; Bacterium smaragdinum 

 Mez, Mikroskopische Wasseranalyse, Ber- 

 lin, 1898, 49; Migula, Svst. d. Bakt., £, 1900, 

 890.) 



sma.rag'di.na. Gr. adj. smaragdinus of 

 smaragdus, emerald -green. 



Small rods, occurring singly. Non -motile. 

 Gram-negative. 



Gelatin colonies: Small, convex, irregular, 

 whitish with greenish shimmer. 



Gelatin stab: Slight surface growth. In- 

 fundibuliform liquefaction. The liquefied 

 medium becomes light emerald-green in 

 color. 



Agar colonies: Small, brownish yellow, 

 convex. 



Agar slant: Abundant growth with green- 

 ish fluorescence. 



Broth: Turbid. 



Litmus milk: Not coagulated. 



Potato: Dark brown, becoming chocolate- 

 brown. 



Indole not produced. 



Nitrates not reduced. 



The cultures give off an odor resembling 

 jasmine. 



Aerobic, facultative. 



Optimum temperature, 37° C. 



Subcutaneous and intravenous inocula- 

 tions into rabbits cause death in 36 to 48 

 hours. 



Source: Isolated from nasal secretions in 

 ozena. 



Habitat: Unknown. 



20. Pseudomonas putida (Trevisan, 

 1889) Migula, 1895. {Bacillus fluorescens 

 putidus Fliigge, Die Mikroorganismen, 2 

 Aufl., 1886, 288; Bacillus putidus Trevisan, 

 I gen. e le specie d. Batteriacee, 1889, 18; 

 Migula, in Engler and Prantl, Die natiir. 

 Pflanzenfam., 1, la, 1895, 29.) 



])u'ti.da. L. adj. putidus stinking, fetid. 



Rods with rounded ends. Motile, posses- 

 sing polar flagella. Gram-negative. 



Gelatin colonies: Small, finely granular, 

 fluorescent with dark center, surrounded by 

 a yellow zone, with pale gray margin. 



Gelatin stab: Dirty white surface growth, 

 becoming greenish, fluorescent. No liquefac- 

 tion. 



Agar colonies: Circular, raised, smooth, 

 amorphous, entire, with fluorescent zone 

 around the periphery. 



Agar slant: Yellowish green layer, be- 

 coming fluorescent. 



Broth: Turbid, fluorescent. 



Litmus milk: Unchanged. 



Potato: Thin, gray to brownish, slim}- 

 layer. 



Cultures give off odor of trimethylamine. 



Indole not produced. 



Nitrites produced from nitrates. 



Aerobic, facultative. 



Temperature relations: Optimum, 25° C. 

 Will grow at 37° C. (Reid et al., Penn. Agr. 

 Exp. Sta., Bull. 422, 1942,9). 



Relationship to other species: Identical 

 with Pseudomonas flxiorescens Migula ac- 

 cording to Lehmann and Neumann (Bact. 

 Diag., 1, Aufl., 2, 1896, 271) except that it 

 does not liquefy gelatin. See Pseudomonas 

 eisenbergii Migula. 



Source: Isolated from putrid materials. 



Habitat: Putrefying materials; water. 



21. Pseudomonas striata Chester, 1901. 

 {Bacillus striatus viridis Ravenel, Memoirs 



