FAMILY IV. PSEUDOMONADACEAE 



109 



Memoirs Nat. Acad. Sci., 7, 1895, 436; 

 Chester, Man. Determ. Bact., 1901, 323.) 



in.cog'ni.ta. L. adj. incognitus not ex- 

 amined, unknown. 



Short rods, with rounded ends, occurring 

 singly, in pairs and in chains. Motile, pos- 

 sessing a polar flagellum. Gram-negative. 



Gelatin colonies: Thin, translucent, 

 slightly granular, becoming greenish. Mar- 

 gin undulate. The medium assumes a blue- 

 green fluorescence. 



Gelatin stab: No liquefaction. 



Agar slant: Thin, moist, translucent. Agar 

 becomes greenish. 



Broth: Turbid, becoming greenish. Pel- 

 licle and whitish sediment form. 



Litmus milk: Slightly acid in a month. 

 Litmus slowly reduced. 



Potato: Moist, glistening, spreading, 

 brown. 



Indole is produced (trace). 



Aerobic, facultative. 



No growth at 35° to 36° C. 



Comment: Wright (op. cit., 1895, 441) 

 described an organism that is very similar 

 to this species except that it may produce a 

 faint brownish green coloration in a gelatin 

 stab; Wright named the organism Bacillus 

 nexibilis {Bacterium nexibilis Chester, Ann. 

 Rept. Del. Col. Agr. Exp. Sta., 9, 1897, 74; 

 Pseudomonas nexibilis Chester, op. cit., 

 1901, 309). 



Source: Isolated from water from the 

 Schujdkill River. 



Habitat: Water. 



25. Pseudomonas rugosa (Wright, 1895) 

 Chester, 1901. {Bacillus rugosus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 438; 

 Chester, Man. Determ. Bact., 1901, 323.) 



ru.go'sa. L. adj. rugosus full of wrinkles. 



Small rods, with rounded ends, occurring 

 singl}^ in pairs and in chains. Motile, pos- 

 sessing 1 to 4 polar flagella. Gram-negative. 



Gelatin colonies: Grayish, translucent, 

 slightly raised, irregular, sinuous, radiately 

 arose to entire. 



Gelatin stab : Dense grayish green, lim- 

 ited, wrinkled, reticulate surface growth. 

 No liquefaction. Medium becomes green. 



Agar slant: Grayish white, limited, 

 slightl}^ wrinkled, translucent. Agar be- 

 comes green. 



Broth: Turbid, with thin whitish pellicle 

 and sediment. 



Litmus milk: Acid, coagulated, partly re- 

 duced. 



Potato: Moist, glistening, brown, spread- 

 ing. 



Indole is produced (trace). 



Aerobic. 



Optimum temperature, 30° C. Does not 

 grow at 35° C. 



Source: From water from the Schuylkill 

 River. 



Habitat: Water. 



26. Pseudomonas mildenbergii Bergey 

 et al., 1930. (Der Blaubacillus, Mildenberg, 

 Cent. f. Bakt., II Abt., 56, 1922, 309; Bergey 

 et al., Manual, 3rd ed., 1930, 172.) 



mil.den.ber'gi.i. Mildenberg, a patro- 

 nymic; M.L. gen. noun mildenbergii of Mil- 

 denberg. 



Rods, 0.3 to 0.5 by 1.0 to 3.5 microns, 

 with rounded ends, occurring singly. Mo- 

 tile, possessing polar flagella. Gram-nega- 

 tive. 



Gelatin colonies: Circular, lobed, smooth, 

 glistening, slightly raised, steel-blue, entire. 



Gelatin stab: No liquefaction. 



Agar colonies: Small, circular, yellowish 

 or reddish yellow, entire, becoming lobed, 

 grayish green, iridescent. The medium be- 

 comes dirty grayish green. 



Agar slant: Smooth, spreading, slimy, 

 glistening, grayish green to dark green, 

 fluorescent. 



Broth: Turbid green, iridescent to opales- 

 cent with slimy sediment. 



Litmus milk: Not coagulated, blue ring. 



Potato: Slimj^, glistening, spreading, steel 

 blue. 



Indole not produced. 



Nitrites not produced from nitrates. 



Aerobic, facultative. 



Optimum temperature, 25° C. 



Source : Isolated from air. 



27. Pseudomonas eonvexa Chester, 

 1901. {Bacillus fluorescens convexus Wright, 

 Memoirs Nat. Acad. Sci., 7, 1895, 438; 

 Chester, Man. Determ. Bact., 1901, 325.) 



con.vex'a. L. adj. convexus vaulted, con- 

 vex. 



Short, thick rods, with rounded ends. 



