no 



PSEUDOMONADALES 



Motile, possessing a polar flagellum. Gram- 

 negative. 



Gelatin colonies: Circular, convex, glis- 

 tening, greenish, translucent. The medium 

 becomes blue-green, fluorescent. 



Gelatin stab: Light green, raised, glisten- 

 ing surface growth. No liquefaction. Me- 

 dium becomes blue-green fluorescent. 



Agar slant: Moist, translucent, glisten- 

 ing, light greenish. The medium assumes a 

 greenish color. 



Broth: Turbid, becoming greenish. 



Litmus milk: No coagulation; alkaline. 



Potato: Pale brown, spreading. 



Indole not produced. 



Aerobic, facultative. 



Little or no growth at 35° to 36° C. 



Source: From water from the Schuylkill 

 River. 



Habitat: Water. 



28. Pseudomonas eisenbergii Migula, 

 1900. (Fluorescirender Bacillus No. 18, 

 Eisenberg, Bakt. Diag., 1 Aufl., 1886, Taf. 

 7; Bacillus fluorescens non liquefaciens Eis- 

 enberg, Bakt. Diag., 3 Aufl., 1891, 145; Mig- 

 ula, Syst. d. Bakt., 3, 1900, 913; Pseud- 

 omonas non-liquefaciens Bergey et al.. 

 Manual, 1st ed., 1923, 132.) 



eis.en.ber'gi.i. Named for James Eisen- 

 berg, the bacteriologist who first described 

 this species; M.L. gen.n. eisenbergii of 

 Eisenberg. 



Short, slender rods with rounded ends. 

 Non-motile. Gram-negative. 



Gelatin colonies: Fern-like surface col- 

 onies. Medium around colonies has a pearly 

 luster. 



Gelatin stab: Surface growth has fluores- 

 cent shimmer. Scant growth along stab. No 

 liquefaction. 



Agar slant: Greenish growth. 



Broth: Turbid, fluorescent. 



Litmus milk: Unchanged. 



Potato: Diffuse, brownish laj-er. The sur- 

 face acquires a grayish blue color. 



Indole not produced. 



Nitrites produced from nitrates. 



Acid from glucose. 



Blood serum liquefied. 



Aerobic. 



Optimum temperature, 25° C. 



Not pathogenic. 

 Habitat : Water. 



29. Pseudomonas erythra Fuller and 

 Norman, 1943. (Jour. Bact., 46, 1943, 276.) 



e'ry.thra. Gr. adj. erythrus red. 



Rods, 0.2 to 0.4 by 1.2 to 1.5 microns, with 

 rounded ends, usually arranged singly. 

 Motile with a single polar flagellum. Encap- 

 sulated. Gram-negative. 



Gelatin stab: No growth. 



Starch agar: No growth. 



Water-insoluble dextrin agar: Scant 

 growth. Subsurface colonies appear after 

 8 to 10 days. Colonies are angular, small, 

 surrounded by a clear zone 2 to 5 mm in 

 diameter. Buff or reddish brown. 



Litmus milk: No growth. 



Indole not produced. 



Nitrites not produced from nitrates. 



Starch not hydrolyzed. 



No growth in media containing the usual 

 carbohydrates. Cellulose and water-in- 

 soluble dextrins are utilized. Filter paper 

 strips in mineral solution develop reddish 

 brown spots above the surface of the liquid. 

 Solution becomes cloudy. Colonies enlarge 

 and become viscous, and the paper becomes 

 reddish. The filter paper does not break 

 with moderate shaking but may be wound 

 up in a slimy mass. In cellulose media a 

 reddish, water-soluble pigment is produced. 



Yeast extract and nitrate are suitable 

 nitrogen sources. 



Aerobic. 



Grows in a range from 22° to 35° C. 



Source: Isolated from soil. 



Habitat: Soil. 



30. Pseudomonas fragi (Eichholz, 1902) 

 Huss, 1907, emend. Hussong et al., 1937. 

 {Bacterium fragi Eichholz, Cent. f. Bakt., 

 II Abt., 9, 1902, 425; Huss, Cent. f. Bakt., 

 II Abt., 19, 1907, 661; Hussong, Long and 

 Hammer, Iowa Agr. Exp. Sta. Res. Bull. 

 225, 1937, 122.) 



fra'gi. L. neut.n. fragum strawberry; L. 

 gen.n. fragi of the strawberry. 



Description from Hussong, Long and 

 Hammer {loc. cit.). 



Rods, 0.5 to 1.0 by 0.75 to 4.0 microns, 

 occurring singly, in pairs and in chains. 



