FAMILY IV. PSEUDOMONADACEAE 



113 



439; Chester, Man. Determ. Bact., 1901, 

 308.) 



am.bi'gu.a. L. adj. anibiguus going 

 about, hence uncertain. 



Small rods, with rounded ends, occurring 

 singly, in pairs and in chains. Motile, pos- 

 sessing a polar flagellum. Gram-negative. 



Gelatin colonies: Gray, translucent, 

 slightly raised, irregular, radiate, with 

 transparent margin. 



Gelatin stab: No liquefaction. 



Agar slant: Graj^, limited, entire. 



Broth: Turbid, with gray sediment. 



Litmus milk: Acid, slowly coagulated. 

 Litmus reduced. 



Potato: Gray to creamj-, viscid, spread- 

 ing. 



Indole produced. 



Aerobic, facultative. 



Optimum temperature, between 30° and 

 35° C. 



Source: Isolated from water from the 

 Schuylkill River. 



Habitat: Water. 



36. Pseudonionas oleovorans Lee and 



Chandler, 1941. (Jour. Bact., 4^, 1941, 378.) 



o.le.o'vor.ans. L. ole^^moil■, L. v. voro to 

 destro}', consume; M.L. part. adj. oleovorans 

 oil-consuming. 



Short rods, 0.5 by 0.8 to 1.5 microns, 

 occurring singly and in pairs. Motile. Gram- 

 negative. 



Gelatin stab: No liquefaction after 6 

 weeks. 



Gelatin colonies : Up to 1 mm in diameter, 

 fluorescent; similar to agar colonies. 



Surface agar colonies: After 24 hours 1 to 

 2 mm in diameter, smooth, convex, shiny, 

 opaque, creamy, fluorescent bj^ transmitted 

 light. Edge entire in young colonies. 



Deep agar colonies: 0.5 by 1.0 to 1.5 mm, 

 lens-shaped, buff-colored, not fluorescent. 



Agar slant: Growth raised, smooth, fluo- 

 rescent, edge erose. 



Broth : After 24 hours, moderate turbidity 

 with slight yellowish, viscid sediment. No 

 pellicle or ring. No soluble pigment pro- 

 duced. 



Litmus milk: No change. 



Indole not produced. 



Potato: Good growth. 



Nitrites are produced from nitrates. 



Starch is hydrol3'zed. 



No acid from glucose, lactose, sucrose, 

 galactose, xylose, mannitol, salicin or 

 glycerol. 



Equally good growth at 25° and 37° C. 



Aerobic. 



Distinctive character: The fluorescent 

 quality of the colonies is not imparted to 

 any of the artificial media used. 



Source: Isolated from cutting compound 

 (oil-water emulsion) circulating in a ma- 

 chine shop. The oil in this compound may 

 be utilized as a sole source of energj'. 



Habitat: Probably oil-soaked soils. Abun- 

 dant in cutting compounds. 



37. Pseudonionas arvilla Gray and 

 Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 

 1928, 90.) 



ar.vil'.la. L. arvum a field; M.L. dim. noun 

 arvilla a small field. 



Rods, 0.5 to 0.7 by 2.0 to 3.0 microns. 

 Motile with one to five polar flagella. Gram- 

 negative. 



Gelatin colonies: Circular, whitish, con- 

 vex, smooth, glistening, lobate. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular or amoeboid, 

 white to buff, flat to convex, smooth, glis- 

 tening, opaque, entire. 



Agar slant: Filiform, whitish, convex, 

 smooth, ringed, entire. 



Broth: Turbid. 



Nitrites not produced from nitrates. 



Starch not hydrolj'zed. 



Acid from glucose. 



Attacks naphthalene. 



Aerobic, facultative. 



Grows at 37° C. 



Source: Isolated from soil. 



Habitat: Soil. 



38. Pseudomonas daciinhae Gray and 

 Thornton, 1928. (Cent. f. Bakt., II Abt., 73, 

 1928, 90.) 



da.cun'hae. d'Acunha, place name. Island; 

 M.L. gen. noun dacimhae of d'Acunha. 



Rods 0.5 to 0.8 by 1.5 to 3.0 microns. 

 Motile with one to six polar flagella. Gram- 

 negative. 



Gelatin colonies: Circular, whitish, 

 raised, smooth, glistening, entire. 



Gelatin stab: No liquefaction. 



