FAMILY IV. PSEUDOMONADACEAE 



115 



Gelatin colonies: Circular, white, convex, 

 smooth, undulate. 



Gelatin stab: No liquefaction. 



Agar colonies: Circular or amoeboid, 

 white to buff, flat to convex, smooth, entire. 



Agar slant: Filiform, pale buff, raised, 

 smooth, undulate. 



Broth: Turbid. 



Nitrites not produced from nitrates. 



Starch not hj^droh-zed. 



No acid in carbohydrate media. 



Attacks phenol and m-cresol. 



Aerobic, facultative. 



Optimum temperature, between 30° and 

 35° C. 



Source: Isolated from soil. 



Habitat: Soil. 



43. Pseudonionas stutzeri (Lehmann 

 and Neumann, 1896) Kluyver, 1942. 

 {Bacillus denitrificans II Burri and Stutzer, 

 Cent. f. Bakt., II Abt., 1, 1895, 392; Bac- 

 terium stutzeri Lehmann and Neumann, 

 Bakt. Diag., 1 Aufl., 2, 1896, 237; Bacillus 

 nitrogenes Migula, Syst. d. Bakt., 2, 1900, 

 793; Pseudonionas stutzeri Kluyver, in 

 Koningsberger, Leerb. d. algem. Plant- 

 kunde, Scheltema and Holkema, Amster- 

 dam, 2, 1942, 198; not Pseudonionas stutzeri 

 Migula, Syst. d. Bakt., 2, 1900, 929.) 



stut'ze.ri. Named for Dr. A. Stutzer, one 

 of the bacteriologists who originally de- 

 scribed this species; M.L. gen. noun stutzeri 

 of Stutzer. 



Description taken from van Niel and 

 Allen (Jour. Bact., 64, 1952, 421). 



Rods, 0.5 to 0.8 by 1.0 to 3.0 microns. Mo- 

 tile, possessing a single polar flagellum. 

 Gram-negative. 



Gelatin and agar colonies: Strongly co- 

 herent to media, dry consistency later be- 

 coming mucoid, resemble craters with ele- 

 vated ridges which often branch and merge, 

 concentric zones, polygonal elements, 

 granular. 



Gelatin: No liquefaction. 



Peptone and yeast agar: Good growth. 



Broth: Surface film on nitrate- or nitrite- 

 free media which readily breaks up and pre- 

 cipitates. 



Potato: Luxuriant, wrinkled, slimy, flesh- 

 to peach-colored growth. 



Nitrates, nitrites, nitramines and N2O 

 reduced to elemental nitrogen. 



Carbohj^drates : No growth when used as 

 a carbon source in mineral media. 



Aerobic, facultative. 



Optimum pH, 7.0; growth even at pH 9.0. 



Optimum temperature, 35° C. 



Distinctive characters: Colony shape 

 and consistency, mode and color of potato 

 growth, ability to grow anaerobically in 

 media with nitrate, nitrite, nitramine or 

 N2O, producing foam. 



Source: Isolated from soil. 



Habitat: Found widely distributed in soil, 

 manure, mud and stagnant water. 



44. Pseudomoiias tralucida Kellerman 

 et al., 1913. (Kellerman, McBeth, Scales 

 and Smith, Cent. f. Bakt., II Abt., 39, 1913, 

 37.) 



tra.lu'ci.da. L. adj. frahicidus trans- 

 parent. 



Rods 0.6 by 1.2 microns. Motile with one 

 or two polar flagella. Gram-negative. 



Gelatin stab: No liquefaction. 



Agar slant: Moderate, flat, glistening, 

 grayish growth. 



Broth: Turbid; granular sediment. 



Litmus milk: Acid, no coagulation. 



Potato: No growth. 



Indole not produced. 



Nitrites produced from nitrates. 



Ammonia not produced. 



Starch hydrolysis slight. 



Acid from glucose, maltose, lactose, su- 

 crose, starch, glycerol and mannitol. 



Attacks cellulose. 



Aerobic, facultative. 



Optimum temperature, 37° C. Grows also 

 at 20° C. 



Habitat: Soil. 



45. Pseudonionas lasia Fuller and Nor- 

 man, 1943. (Jour. Bact., 46, 1943, 275.) 



la'si.a. Gr. adj. lasius hairy, rough, 

 shaggy, woolly. 



Rods, 0.5 to 0.6 by 1.2 to 2.0 microns, 

 usually occurring singly but sometimes in 

 chains. Motile with a single polar flagellum. 

 Gram-negative. 



Gelatin stab: No liquefaction. 



Starch agar colonies : Convex, pale 3'ellow, 

 becoming cream color, entire, round. Sub- 



