FAMILY IV. PSEUDOMONADACEAE 



119 



lophotrichous flagella. Encapsulated. Gram- 

 negative. 



Gelatin stab : Growth filiform, best at top, 

 1 with slow crateriform liquefaction. 



Agar colonies: Circular, 1.0 to 2.5 mm, 

 with crinkled surface. 



Agar slant: Moderate, beaded, raised 

 growth. ]\Iembranous consistency. Becomes 

 browned with age. 



Broth: Slight turbidity, flocculent sedi- 

 ment, film of growth on walls of test tube. 



IMilk: No growth. 



Potato: No growth. 



Indole not produced. 



Nitrites not produced from nitrates. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, sucrose, 

 dextrin and mannitol. No acid from lactose 

 or xylose. 



Starch not hydrolyzed. 



Optimum temperature, between 20° and 

 25° C. 



Aerobic. 



Source: Isolated from sea water. 



Habitat: Sea water. 



54. Pseudonionas gelatica (Gran, 1902) 

 Bergey et al., 1930. {Bacillus gelaticus Gran, 

 Bergens Museums Aarbog., 1902, 14; Bergey 

 et al.. Manual, 3rd ed., 1930, 175.) 



ge.la'ti.ca. L. part. adj. gelatns frozen, 

 congealed, jellied; M.L. adj. gelaticus re- 

 sembling hardened gelatin. 



Rods, with rounded ends, 0.6 to 1.2 by 1.2 

 to 2.6 microns, occurring singly, in pairs, 

 and sometimes in short chains. Motile, type 

 of flagellation not recorded. Gram -negative. 



All media prepared with 3 per cent salt. 



Fish-gelatin colonies: Circular, trans- 

 parent, glistening, becoming brownish in 

 color. 



Fish-gelatin stab: Liquefaction infun- 

 dibuliform. Two varieties are recognized: 

 one produces a green fluorescence; the other 

 does not produce a water-soluble pigment. 



Sea-weed agar colonies: Circular, flat, 

 entire, glistening, grayish blue center with 

 reddish brown periphery. Liquefied. 



Fish-agar slant: Flat, transparent streak, 

 with undulate margin, reddish brown to 

 grayish white. 



Broth: Turbid with flocculent pellicle 

 and grayish yellow sediment, viscid. 



Indole not produced. 



Nitrites not produced from nitrates. 



Starch hj^drolyzed. 



No action on sugars. 



Aerobic, facultative. 



Temperature relations : Optimum temper- 

 ature, between 20° and 25° C. Maximum, 

 between 30° and 32° C. Minimum, 0° C. 



Distinctive character: Requires 3 to 4 per 

 cent salt for growth. 



Source : Isolated from sea water from the 

 Norwegian coast. 



Habitat: Probably associated with the de- 

 composition of algae in coastal waters. 



55. Pseudomonas calcis (Drew, 1912) 

 Kellerman and Smith, 1914. (Bacterium 

 calcis Drew, Yearbook Carnegie Inst. 

 Wash., 11, 1912, 136; Kellerman and Smith, 

 Jour. Wash. Acad. Sci., 4, 1914, 400.) 



cal'cis. L. fem.noun calx, calcis limestone, 

 chalk; L. calcis of limestone. 



Rods, 1.1 by 1.5 to 3.0 microns, usually 

 single but may form long chains. Actively 

 motile with one polar flagellum. Gram- 

 negative. 



Grows best in sea water or 3 per cent salt 

 media. Deposits CaCOs . 



Agar colonies: Circular, with finely ir- 

 regular outline, granular appearance, ele- 

 vated, spreading; old colonies having 

 brownish tinge in center. 



Gelatin stab: Infundibuliform liquefac- 

 tion. 



Gelatin colonies : Small, with liquefaction. 



Broth: Good growth especially in pres- 

 ence of potassium nitrate, peptone or cal- 

 cium malate. 



Acid from glucose, mannitol and sucrose 

 but not from lactose. 



Nitrates reduced to nitrites and am- 

 monia. 



Aerobic, facultative. 



Optimum temperature, between 20° and 

 28° C. 



Habitat: Sea water and marine mud. 



56. Pseudomonas calciprecipitans 



Molisch, 1925. (Cent. f. Bakt., II Abt., 65, 

 1925, 130.) 



cal.ci. pre. ci'pi. tans. L. calx, calcis lime; 

 L. praecipito to throw down; M.L. part. adj. 

 calciprecipitans lime-precipitating. 



