FAMILY IV. PSEUDOMONADACEAE 



121 



Salt range: 0.25 to 6.0 percent. Optim\im, 

 1.0 to 4.0 per cent. 



Source: Sea water. 



Habitat: Common along the coast of the 

 North Pacific. 



58. Pseudomonas beijerinckii Hof, 

 1935. (Travaux botaniques neerlandais, 32, 

 1935, 152.) 



bei.jer.inck'i.i. M.L. gen. noun heije- 

 rinckii of Beijerinck; named for Prof . M. W. 

 Beijerinck of Delft, Holland. 



Small rods. Motile with polar flagelhi. 



Gelatin: No liquefaction. 



Indole not produced. 



Nitrites produced from nitrates by four 

 out of six strains. 



Cellulose not decomposed. 



Acid from glucose. In yeast-water with 2 

 per cent glucose and 12 per cent NaCI, no gas 

 is produced. 



Pigment production: Insoluble purple pig- 

 ment produced but not in all media; is lo- 

 calized markedl}^; reduced oxygen tension 

 necessary; optimum pH, 8.0; not produced 

 in yeast-water or in peptone-water; pro- 

 duced only when grown in extracts of beans 

 or some other vegetable. 



Aerobic. 



Source: Six strains isolated from beans 

 preserved with salt. 



Habitat: Causes purple discoloration of 

 salted beans. 



59. Pseudomonas aceris (Ark, 1939) 

 Starr and Burkholder, 1942. (Phytomonas 

 aceris Ark, Phytopath., 29, 1939, 969; Starr 

 and Burkholder, Phytopath., 32, 1942, 601.) 



a'ce.ris. L. acei- the maple; L. neut. 

 gen. noun aceris of the maple. 



Rods 0.3 to 0.8 by 0.8 to 2.5 microns. 

 Motile, with 1 to 2 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced. 



Gelatin: Liquefied. 



Beef -extract-peptone agar: Colonies are 

 grayish white, appearing in 24 hours. 



Broth: Turbid. 



Milk: Clearing with no coagulation. 



Nitrites produced from nitrates (Burk- 

 holder and Starr, Phytopath., 38, 1948, 498). 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid from glucose, fructose, galactose, 

 arabinose, xylose, sucrose, maltose, lactose, 

 raffinose, mannitol, glycerol and dulcitol. 



Slight growth in broth plus 6 per cent salt 

 (Burkholder). 



Optimum temperature, between 13° and 

 31° C. 



Source : From diseased leaves of the large 

 leaf maple, Acer niacrophyllum. 



Habitat: Causes a disease of Acer spp. 



60. Pseudomonas angulata (Fromme 

 and Murray, 1919) Holland, 1920. (Bac- 

 terium angulatum Fromme and Murray, 

 Jour. Agr. Res., 16, 1919, 219; Holland, Jour. 

 Bact.,5, 1920,224.) 



ang.u.la'ta. L. part. adj. angulatus wdth 

 angles, angular. 



Description from Clara (Cornell Agr. 

 Exp. Sta. Mem. 159, 1934, 24). 



Rods 0.75 to 1.5 by 1.5 to 3.0 microns. 

 Motile, with 1 to 6 polar flagella. Gram- 

 negative. 



Gelatin: Liquefied. 



Green fluorescent pigment produced. 



Beef-extract agar colonies: Dull white, 

 circular, raised, smooth and glistening. 



Broth: Turbid and greenish in 36 hours. 



Milk: Alkaline. 



Nitrites produced from nitrates (Burk- 

 holder and Starr, Phytopath., 38, 1948, 498). 



Indole not produced. 



Hydrogen sulfide not produced. 



Lipolytic action negative (Starr and 

 Burkholder, Phytopath., 32, 1942, 601). 



Acid but no gas from glucose, galactose, 

 fructose, mannose, arabinose, xylose, su- 

 crose and mannitol. Alkaline reaction from 

 salts of citric, malic, succinic and tartaric 

 acids. Rhamnose, maltose, lactose, raffinose, 

 glycerol, salicin, and acetic, lactic and 

 formic acids are not fermented. 



Starch not hydrolyzed. 



Slight growth in broth plus 5 to 6 per cent 

 salt (Burkholder). 



Aerobic, facultative. 



Relationship to other species: Braun 

 (Phytopath., 27, 1937, 283) considers this 

 species to be identical in culture with Pseu- 

 domonas tabaci, but they differ in the type of 

 disease they produce. 



Source : Isolated by Fromme and Murray 

 from small angular leaf spots on tobacco. 



