122 



ORDER I. PSEUDOMONADALES 



Habitat : Causes the angular leaf spot of 

 tobacco {Nicotiana tahacum). 



61. Pseudomonas aptata (Brown and 

 Jamieson, 1913) Stevens, 1925. {Bacterium 

 aptatum Brown and Jamieson, Jour. Agr. 

 Res., 1, 1913, 206; Stevens, Plant Disease 

 Fungi, New York, 1925, 22.) 



ap.ta'ta. L. part. adj. aptahis adapted. 



Rods 0.6 by 1.2 microns. Motile, with bi- 

 polar fiagella. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Agar slants: Moderate growth along 

 streak, filiform, whitish, glistening. 



Broth: Turbid, with formation of a pel- 

 licle. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole not produced in 10 daj's. Slight 

 amount found later. 



Hydrogen sulfide not produced. 



Acid from glucose, galactose and sucrose. 

 No acid from lactose, maltose or mannitol 

 (Paine and Banfoot, Ann. Appl. Biol., 11, 

 1924, 312). 



Starch not hydrolyzed. 



Slight growth in broth plus 7 per cent salt 

 (Burkholder) . 



Temperature relations: Optimum, be- 

 tween 27° and 28° C. Minimum, below 1° C. 

 Maximum, between 34° and 35° C. 



Aerobic. 



Source : Isolated from diseased nasturtium 

 leaves from Virginia and diseased beet 

 leaves from Utah. 



Habitat: Pathogenic on sugar beets, nas- 

 turtiums and lettuce. 



62. Pseudomonas primulae (Ark and 

 Gardner, 1936) Starr and Burkholder, 1942. 

 {Phytomonas primnlae Ark and Gardner, 

 Phytopath., S6, 1936, 1053; Starr and Burk- 

 holder, Phytopath., 32, 1942, 601.) 



pri'mu.lae. L. dim. adj. prinmlus the first; 

 M.L. fem.noun Primula generic name; 

 M.L. gen. noun primulae of Primula. 



Rods 0.51 to 0.73 by 1.0 to 3.16 microns. 

 Motile, with a polar flagellum. Gram-nega- 

 tive. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Agar colonies: Round, convex, smooth, 

 glistening, j-ellowish. 



Milk: Coagulated. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolj'tic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid but no gas from glucose, lactose, su- 

 crose, maltose, galactose, arabinose, glyc- 

 erol, dulcitol and mannitol. Starch not 

 hydrolyzed. 



Growth in broth plus 5 per cent salt. 



Temperature relations : Optimum between 

 19° and 22° C. Minimum, 10° C. Maximum, 

 34° C. 



Optimum pH between 6.8 and 7.0. Mini- 

 mum, between 4.5 and 5.0. 



Aerobic, facultative. 



Source : Isolated from leaf -spot of Primula 

 polyantha. 



Habitat: Pathogenic on Primula spp. 



63. Pseudomonas viridilivida (Brown, 

 1915) Holland, 1920. {Bacterium viridilivi- 

 dum Brown, Jour. Agr. Res., 4, 1915, 475; 

 Holland, Jour. Bact., 5, 1920, 225.) 



vi.ri.di.li'vi.da. L. viridis green; L. livi- 

 dus blue; M.L. adj. viridilividus greenish 

 blue. 



Rods 1.0 to 1.25 by 1.25 to 3.0 microns. 

 Motile, with 1 to 3 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Slow liquefaction. 



Beef agar colonies: Cream- white, round, 

 smooth, translucent, edges entire. 



Broth: Turbid, becomes lime-green. 



Milk: Alkaline and clears. 



Nitrites produced from nitrates (Burk- 

 holder and Starr, Phytopath., 38, 1948, 498). 



Indole produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., S^, 1942,601). 



Acid from glucose and sucrose (Burk- 

 holder). 



Grows well in 4.5 per cent salt. Grows in 7 

 per cent salt (Burkholder). 



Temperature relations: Minimum, 1.5° C. 

 Maximum, 34.5° C. 

 Aerobic. 



