124 



ORDER I. PSEUDOMONADALES 



glistening, smooth, edges entire. Grayish 

 white with bluish tinge. 



Broth: Turbid in 36 hours. Pellicle 

 formed. 



Milk: Becomes alkaline. No curd. 



Nitrites produced from nitrates (Burk- 

 holder and Starr, Phytopath., 38, 1948, 498). 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, galactose, 

 fructose, mannose, arabinose, xylose, su- 

 crose, mannitol and glycerol. Alkaline reac- 

 tion from salts of acetic, citric, malic and 

 succinic acids. Rhamnose, maltose, lactose, 

 raffinose salicin, and formic, lactic and 

 artaric acids are not utilized. 



Starch not hydrolyzed. 



Aerobic, facultative. 



Distinctive character: Pathogenicity ap- 

 pears limited to celery. 



Source: Jagger isolated this repeatedly 

 from diseased celery leaves. 



Habitat: Pathogenic on celery, Apiutn 

 graveolens. 



67. Pseudomonas asplenii (Ark and 

 Tompkins, 1946) Savulescu, 1947. (Phj/to- 

 monas asplenii Ark and Tompkins, Phyto- 

 path., 36, 1946, 760; Savulescu, Anal. Acad. 

 Romane, III, 2^, 1947, 11.) 



a.sple'ni.i. Gr. neut.noun asplenum 

 spleenwort; M.L. neut.noun Asplenium 

 generic name; M.L. gen.noun asplenii of 

 Asplenium. 



Rods 0.3 to 0.5 by 1.2 to 2.4 microns. 

 Motile, with 1 to 3 polar flagella. Gram- 

 negative. 



Gelatin: Liquefied. 



Beef -extract-peptone agar slants : Grayish 

 white with fluorescence in the medium. 



Potato-dextrose-peptone agar: Growth 

 rapid, heavy, strongly grayish white, 

 butyrous; medium darkens with age. 



Nutrient broth: Turbid in 24 hours; no 

 pellicle. 



Milk: No curd. 



Indole not produced. 



Nitrites not produced from nitrates. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, galactose, 

 fructose, arabinose, xylose, maltose and 

 sucrose. Slight acidity in lactose after long 

 incubation; no acid in rafiinose. 



Starch not hydrolyzed. 



Growth good in Fermi's, Cohn's and 

 Uschinsky's solutions. 



Temperature relations : Optimum between 

 22° and 30° C. Minimum, 1° C. Maximum, 

 34° C. 



Source : Six isolates and 3 reisolates from 

 lesions on the bird's nest fern. 



Habitat: Pathogenic on the fern, Asple- 

 nium nidus. 



68. Pseudomonas berberidis (Thorn- 

 berry and Anderson, 1931) Stapp, 1935. 

 (Phytomonas berberidis Thornberry and An- 

 derson, Jour. Agr. Res., 43, 1931, 36; Stapp, 

 Bot. Rev., 1, 1935,407.) 



ber.be'ri.dis. M.L. Berberis generic name 

 of barberry; M.L. fem. gen. noun berberidis 

 of barberry. 



Rods, 0.5 to 1.0 by 1.5 to 2.5 microns, oc- 

 curring singly or in pairs. Motile with 2 to 4 

 polar flagella. Encapsulated. Gram-negative 

 (Burkholder); not Gram-positive as stated 

 in original description. 



Green fluorescent pigment produced in 

 culture (Burkholder). 



Gelatin: Not liquefied. 



Glucose agar slants: Growth moderate, 

 filiform at first, later beaded, raised, 

 smooth, white. Butyrous in consistency. 



Milk: Becomes alkaline. No other change. 



Nitrites produced from nitrates (Burk- 

 holder and Starr, Phytopath., 38, 1948, 498). 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 3^, 1942, 601). 



Acid from glucose, galactose and sucrose. 

 Maltose and rhamnose not utilized (Burk- 

 holder). 



No gas from carbohydrates. 



Starch not hydrolyzed. 



Temperature relations: Optimum, 18° C. 

 ]\Iinimum, 7° C. Maximum, 30° C. 



Aerobic. 



Source: Repeated isolations from leaves 

 and twigs of barberry. 



Habitat: Pathogenic on barberry, Berberis 

 thnnbergerii and B. vulgaris. 



69. Pseudomonas coronafaeiens (El- 

 liott, 1920) Stevens, 1925. {Bacterium corona- 



