FAMILY IV. PSEUDOMONADACEAE 



125 



faciens Elliott, Jour. Agr. Res., 19, 1920, 153; 

 Steven.s, Plant Disease Fungi, 1925, 27.) 



co.ro.na.fa'ci.ens. L. corona crown; L. 

 facio to make; M.L. part. adj. coronafaciens 

 halo-producing. 



Rods, 0.65 by 2.3 microns, occurring in 

 chains. Motile with polar flagella. Encapsu- 

 lated. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Slow liquefaction. 



Nutrient agar colonies: White, becoming 

 irregularly circular, flat with raised mar- 

 gins. 



Broth : Slight turbidity in 24 hours. Heavy 

 pellicle formed. 



Milk: Alkaline. A soft curd formed fol- 

 lowed by clearing. Curd sometimes absent. 



Test for nitrites produced in nitrate broth 

 negative or faint (Burkholder and Starr, 

 Phytopath., 38, 1948, 498). 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid but no gas from glucose and sucrose. 

 Starch hj^drolysis slight. 



Slight growth in broth plus 2 per cent salt. 



Temperature relations: Optimum between 

 24° and 25° C. Minimum, 1° C. Maximum, 

 31° C. 



Comment: A variety pathogenic on 

 brome-grass, Bromus inermis, has been de- 

 scribed by Reddy and Godkin (Phytopath., 

 IS, 1923, 81). Produces water-soaked spots 

 which are dark purple in color. Has been 

 artificially inoculated on oats {Avena saliva) . 

 Also pathogenic on Agropyron repens. 



Source: Numerous isolations from 

 blighted blades of oats. 



Habitat: Causes a halo spot on oats 

 {Avena saliva). Artificial inoculations show 

 barley (Hordeum vulgare), rye (Secale 

 cereale) and wheat {Trilicum aesHvum) to be 

 susceptible. 



70. Pseudotnonas lachrymans (Smith 

 and Bryan, 1915) Carsner, 1918. {Bacterium 

 lachrymans Smith and Bryan, Jour. Agr. 

 Res., 5, 1915, 466; Carsner, Jour. Agr. Res., 

 15, 1918, 201.) 



lach'ry.mans. L. lacrimo to shed tears; 

 M.L. part. adj. lachrymans shedding tears. 



Description from Smith and Bryan {op. 

 cit., 1915, 466) and Clara (Cornell Agr. Exp. 

 Sta. Mem. 159, 1934,26). 



Rods 0.8 by 1.0 to 2.0 micron.s. Motile 

 with 1 to 5 polar flagella. Encapsulated. 

 Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Beef-peptone agar colonies: Circular, 

 smooth, glistening, transparent, whitish, 

 entire margins. 



Broth: Turbid in 24 hours. White precipi- 

 tate with crystals. 



Milk: Turns alkaline and clears. 



Nitrites not produced from nitrates. 



Indole reaction weak. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, Phj-- 

 topath.,5j?, 1942, 601). 



Acid but no gas from glucose, fructose, 

 mannose, arabinose, xylose, sucrose and 

 mannitol. Alkaline reaction from salts of 

 citric, malic and succinic acids. Maltose, 

 rhamnose, lactose, raffinose, glycerol and 

 salicin not fermented (Clara, op. cil., 1934, 

 26). 



Starch partially digested. Not digested 

 (Clara, loc. cit.). 



Growth in 3 per cent salt after 12 days. No 

 growth in 4 per cent salt. 



Temperature relations : Optimum between 

 25° and 27° C. Minimum, 1° C. Maximum, 

 35° C. 



Aerobic, facultative (Clara, loc. cit.). 



Source : Isolated from diseased cucumber 

 leaves collected in New York, Wisconsin, 

 Indiana and in Ontario, Canada. 



Habitat: Pathogenic on cucumber, Cucu- 

 mis sativus, and related plants. 



71. Pseudomonas niaculicola (McCul- 

 loch, 1911) Stevens, 1913. {Baclerium macu- 

 licolum McCulloch, U. S. Dept. Agr., Bur. 

 Plant Ind. Bui., 225, 1911, 14; Stevens, The 

 Fungi which cause Plant Diseases, 1913, 28.) 



ma.cu.li'co.la. L. macula spot; L. -cola a 

 dweller; M.L. noun maculicola spot dweller. 



Rods 0.9 by 1.5 to 3.0 microns. Filaments 

 present. Motile with 1 to 5 polar flagella. 

 Gram-negative. 



