126 



ORDER I. PSEUDOMONADALES 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Beef -peptone agar colonies: Whitish, cir- 

 cular, shining, translucent, edges entire. 



Broth: Turbid. No ring or pellicle. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole production feeble. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid from glucose, galactose, xylose, su- 

 crose, glycerol and mannitol. Alkaline reac- 

 tion from salts of citric, malic, malonic and 

 succinic acids. Salicin, maltose and salts of 

 hippuric and tartaric acids not utilized 

 (Burkholder). 



Slight growth in broth plus 4 per cent salt 

 (Erw. Smith, Bact. Plant Diseases, 1920, 

 306). 



Aerobic. 



Temperature relations : Optimum between 

 24° and 25° C. Minimum, 0° C. Maximum, 

 29° C. 



Source: Isolated from diseased cauliflower 

 leaves from Virginia. 



Habitat: Pathogenic on cauliflower and 

 cabbage. 



72. Pseudonionas mangiferaeindicae 



Patel et al., 1948. (Pseudonionas mangijerae- 

 indicae (sic) Patel, Moniz and Kulkarni, 

 Curr. Sci., 17, 1948, 189; Indian Phytopath., 

 1, 1948, 147.) 



man.gi'fe.rae.in"di.cae. M.L. fem.noun 

 Mangifera mango bearer; L. adj. indicus of 

 Indm; mangiferaeindicae of Mangifera indica. 



Rods, 0.36 to 0.54 by 0.45 to 1.44 microns, 

 occurring singly or in chains of 2 to 4. Motile 

 with 1 or 2 polar flagella. Gram-negative. 



Gelatin: Liquefied. 



Nutrient agar colonies: Flat, smooth, 

 glistening, round with entire margins, white 

 to creamy, border deeper in color. 



Potato glucose agar slants: Growth 

 copious, raised, smooth, glistening, filiform, 

 opalescent, butyrous, white. 



Broth: Turbid with pellicle in 7 days. 

 Slight sediment. 



Milk: Litmus reduced in 7 days. Cleared 

 with gelatinous sediment. Casein digested. 



Loeffler's blood serum: Liquefied. 



Uschinsky's solution: Good growth. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide produced. 



Acid but no gas from glucose, lactose and 

 sucrose. Slight growth and acid in mannitol. 

 L-arabinose, maltose, fructose, inulin, 

 glycerol, salicin, sodium tartrate and 

 asparagine not utilized. 



Starch hydrolyzed. 



Lipase not produced. 



Growth in 2 per cent salt. 



Temperature relations : Optimum between 

 20° and 25° C. Minimum, 5° C. Maximum, 

 35° C. 



Aerobic. 



Source: Isolated from diseased leaves of 

 mangoes. 



Habitat : Pathogenic on Mangifera indica, 

 Spondias mangiferae and Anacardium occi- 

 dentale. 



73. Pseudonionas marginata (McCul- 



loch, 1921) Stapp, 1928. {Bacterium margi- 

 natum McCulloch, Science, 54, 1921, 115; 

 Jour. Agr. Res., 29, 1924, 174; Stapp, in 

 Sorauer, Handbuch der Pflanzenkrank- 

 heiten, 2, 5 Aufl., 1928, 56.) 



mar.gi.na'ta. L. margino to furnish with 

 a border; L. part. adj. marginatus margined. 



Rods 0.5 to 0.6 by 0.8 to 1.8 microns. Mo- 

 tile with 1 to 4 bipolar flagella. Encapsu- 

 lated. Gram-negative. 



Green fluorescent pigment produced in 

 Uschinsky's and Fermi's solutions. 



Gelatin: Liquefied. 



Agar colonies: White, circular, smooth, 

 translucent, viscid, with definite margins at 

 first thin but later thick and contoured. 

 Surface wrinkled. 



Milk: At first slightly acid, then alkaline. 

 Casein digested. 



Nitrites not produced from nitrates. 



Indole production slight. 



Hydrogen sulfide production slight. 



Lipolytic (Starr and Burkholder, Phyto- 

 path., 32, 1942, 601). 



Acid but no gas from glucose, lactose, su- 

 crose and glycerol. 



Starch hydrolysis feeble. 



Growth in 3.5 per cent salt. No growth in 

 4 per cent salt. 



Temperature relations : Optimum between 



