FAMILY IV. PSEUDOMONADACEAE 



127 



30° and 32° C. Minimum between 8° and 

 9° C. Maximum, 40° C. 



pH range, 4.6 to 9.1. 



Source : Repeatedly isolated from diseased 

 gladiolus. 



Habitat: Pathogenic onGladiolus spp. and 

 7m spp. 



74. Pseudonionas inedicaginis Sackett, 

 1910. (Science, 31, 1910, 553; also Colorado 

 Agr. Exp. Sta., Bull. 158, 1910, 11.) 



me.di.ca'gi.nis. Gr. medice the Median 

 grass, alfalfa, lucerne, medic; M.L. fern, 

 noun Medicago generic name of alfalfa; M.L. 

 fem. gen. noun medicaginis of lucerne or 

 alfalfa. 



Rods 0.7 by 1.2 microns. Motile with 1 to 

 4 flagella. Filaments present. Gram-nega- 

 tive. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Not liquefied. 



Nutrient agar colonies: Growth in 24 

 hours whitish, glistening. 



Broth: Turbid in 24 hours. Pellicle 

 formed. Viscid sediment. 



Milk: Becomes alkaline. No change. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 3£, 1942, 601). 



Starch not hydrolyzed. 



No gas from carbohydrates. Acid from 

 sucrose. 



Slight growth in broth plus 3.75 per cent 

 salt. 



Temperature relations : Optimum between 

 28° and 30° C. Maximum, 37.5°C. 



Aerobic. 



Source: Isolated from brown lesions on 

 leaves and stems of alfalfa. 



Habitat: Pathogenic on alfalfa, Medicago 

 sp. 



75. Pseudomonas phaseolicola (Burk- 

 holder, 1926) Dowson, 1943. (Phytomonas 

 medicaginis var. phaseolicola Burkholder, 

 Phytopath., 16, 1926, 915; Dowson, Trans. 

 Brit. Mycol. Soc, 26, 1943, 10.) 



pha.se.o.li'co.la. Gr. phaseolus the kid- 

 ney bean; L. dim. mas. noun phaseolus the 

 kidney bean; L.mas.gen.noun phaseoli of the 



bean; L. cola dweller; M.L. fem. noun 

 phaseolicola the bean dweller. 



Description from Burkholder and Zaleski 

 (Phytopath., ^^, 1932, 85). 



Rods 1.0 by 2.0 microns, sometimes 

 slightly curved; filaments present. Motile 

 with a polar flagellum. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin stab: Slow liquefaction. 



Beef extract agar: Whitish, circular 

 colonies, 2 mm in diameter. Edges entire. 



Broth: Turbid. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 82, 1942, 601). 



Acid but no gas from glucose, fructose, 

 mannose, arabinose, xylose, sucrose and 

 glycerol. No acid from rhamnose, lactose, 

 maltose, mannitol or salicin. Alkali from 

 salts of citric and malic acids, but not from 

 acetic, formic, lactic or tartaric acids. 

 Starch and cellulose not hydrolyzed. 



Slight growth in broth plus 4 per cent salt. 



Temperature relations : Optimum between 

 20° and 23° C. Minimum, 2.5° C. Maximum, 

 33° C. (Hedges, Jour. Agr. Res., 36, 1928, 

 428). 



Chemical tolerance: Optimum pH be- 

 tween 6.7 and 7.3. Minimum between 5.0 

 and 5.3. Maximum between 8.8 and 9.2 

 (Kotte, Phyt. Zeitsch., 2, 1930 453). 



Microaerophilic. 



Source: Isolated from leaves, pod and 

 stem of beans showing halo blight. 



Habitat: Pathogenic on beans (Phaseo- 

 lus vidgaris), the kudzu vine (Pueraria 

 hirsuta) and related plants. 



76. Pseudonionas pisi Sackett, 1916. 

 (Colorado Agr. Exp. Sta., Bull. 218, 1916, 

 19.) 



pi'si. Gr. pisus or pisum the pea; M.L. 

 neut.noun Pisum generic name of the pea; 

 M.L. neut. gen. noun pisi of the pea. 



Rods 0.68 to 2.26 microns. Motile with a 

 polar flagellum. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



