128 



ORDER I. PSEUDOMONADALES 



Agar slants : Moderate growth in 24 hours, 

 filiform, glistening, grayish white. 



Broth: Turbid with a scum in 5 days. 



Milk: Alkaline, soft curd, clears. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hj'drogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid but no gas from glucose, galactose 

 and sucrose. 



Starch not hydrolyzed. 



Temperature relations : Optimum between 

 27° and 28° C. Minimum, 7° C. Maximum, 

 37.5° C. 



Aerobic. 



Source: Ten cultures isolated from 5 col- 

 lections of diseased peas showing water- 

 soaked lesions on stems and petioles. 



Habitat: Pathogenic on garden peas, 

 Pisum sativum, and field peas, P. sativum 

 var. arvense. 



77. Pseudonionas syringae van Hall, 

 1902. (Kennis der Bakt. Pflanzenziekte, 

 Inaug. Diss., Amsterdam, 1902, 191.) 



sy.rin'gae. Gr. syrinx, syringis a pipe or 

 tube; M.L. fem.noun Syringa generic name 

 of syringa or lilac; M.L. fem. gen. noun. 

 syringae of the lilac. 



Description from Clara (Cornell Agr. 

 Exp. Sta. Mem. 159, 1934, 29). 



Rods 0.75 to 1.5 by 1.5 to 3.0 microns. 

 Motile with 1 or 2 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Beef -extract agar colonies: Circular, 

 grayish white with bluish tinge. Surface 

 smooth. Edges entire or irregular. 



Broth: Turbid in 36 hours. No pellicle. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 3^, 1942, 601). 



Slight growth in broth plus 4 per cent salt. 



Acid but no gas from glucose, galactose, 

 mannose, arabinose, xylose, sucrose, man- 

 nitol and glycerol. Alkaline reaction from 

 salts of citric, malic, succinic and lactic 



acids. Rhamnose, maltose, lactose, raffinose, 

 salicin, and acetic, formic and tartaric acids 

 not fermented. 



Starch not hydrolj'zed. 



Aerobic, facultative. 



Comment: Orsini (Intern. Bull. Plant 

 Protect., S3, 1942, 33) reports that a variety 

 of this species is pathogenic on the pepper 

 plant {Capsicum). 



Source: Van Hall originally isolated this 

 pathogen from lilac. 



Habitat: Pathogenic on lilac, citrus, cow 

 peas, beans, lemons, cherries and many un- 

 related plants. 



78. Pseudonionas tomato (Okabe, 1933) 

 Alstatt, 1944. {Bacterium tomato Okabe, 

 Jour. Soc. Trop. Agr. Formosa, 5, 1933, 32; .„ 

 Alstatt, U. S. Dept. Agr., Plant Dis. Rept., 

 28, 1944, 530.) 



to.ma'to. Am.Ind. tomatl; Sp. tornate; 

 Eng. tomato; M.L. noun tomato. 



Rods 0.69 to 0.97 by 1.8 to 6.8 microns. 

 Motile with 1 to 3 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Slow liquefaction. 



Beef -extract agar colonies: White, circu- 

 lar, flat and glistening. 



Broth: Turbid in 24 hours. Pellicle. 



Milk: Becomes alkaline and clears. 



Nitrites are usually produced from ni- 

 trates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, sucrose and 

 lactose. No acid from maltose or glycerol. 



Starch hydrolysis feeble. 



Slight growth in 3 per cent salt. 



Temperature relations : Optimum between 

 20° and 25° C. Maximum, 33° C. 



Aerobic. 



Source: Isolated from diseased tomato 

 leaves. 



Habitat: Pathogenic on tomato, Lyco- 

 persicon esculenium. 



79. Pseudomonas atrofaciens (McCul- 

 loch, 1920) Stevens, 1925. {Bacterium atro- 

 faciens McCulloch, Jour. Agr. Res., 18, 1920, 

 549; Stevens, Plant Disease Fungi, New 

 York, 1925, 22.) 



