FAMILY IV. PSEUDOMONADACEAE 



133 



Rods 1.2 by 3.3 microns. Motile with a 

 polar flagellum. Gram-negative. 



Gelatin: Liquefied. 



Potato agar colonies: Grayish white, cir- 

 cular, raised, wet-shining, smooth. 



Milk: Alkaline; clears. 



Nitrites produced from nitrates (Burk- 

 holder and Starr, Phytopath., 38, 1948, 498). 



Indole not produced. 



Acid from glucose, galactose, fructose, 

 1-arabinose, xj'lose, sucrose, pectin, man- 

 nitol and glycerol (Braun, Phytopath., 27, 

 1937, 289). 



Ammonium sulfate, potassium nitrate, 

 cystine, glutamic acid, glycine, succin- 

 imide, oxamide, acetamide and urea can be 

 used as nitrogen sources (Braun). 



Starch not hydrolyzed. 



Aerobic. 



Relationship to other species: Braun {loc. 

 cit.) states that Pseudomonas tabaci and 

 Pseiidomonas angulata are identical in 

 culture. 



Source: Isolated from wildfire lesions and 

 tobacco leaves in North Carolina. 



Habitat: Pathogenic on tobacco, Nico- 

 tinna fabacvm. 



92. Pseudoniona.s poly color Clara, 1930. 

 (Phytopath., 20, 1930, 704.) 



po.lj^'co.lor. Gr. poly- many; L. color 

 color; M.L. adj. polycolor many colored. 



Description taken from Clara (Cornell 

 Agr. E.xp. Sta. Mem. 159, 1934, 28). 



Rods 0.75 to 1.2 by 1.05 to 3.0 microns. 

 Motile with 1 or 2 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Beef -extract agar colonies: Graj^ish 

 white, circular, raised; thin, transparent 

 margins. 



Broth: Turbid in 36 hours with thin pel- 

 licle. 



Milk: Alkaline; no curd. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Lipolytic (Starr and Burkholder, Phyto- 

 path., 32, 1942, 601). 



Acid l)Ut no gas from glucose, galactose. 



fructose, mannose, arabino.se, xylose, man- 

 nitol and glycerol. Alkaline reaction from 

 salts of acetic, citric, malic, lactic and for- 

 mic acids. Rhamnose, sucrose, maltose, lac- 

 tose, raffinose and salicin not fermented. 



Starch not hydrolyzed. 



Aerobic, facultative. 



Good growth in broth plus 7 per cent salt. 



Temperature relations: Optimum be- 

 tween 25° and 30° C. Maximum between 37° 

 and 39° C. 



Distinctive characters: Differs from 

 Pseudomonas mellea in type of lesion pro- 

 duced; does not digest starch nor reduce 

 nitrates and does not form acid from lactose 

 nor sucrose. Pathogenic for laboratory ani- 

 mals (Elrod and Braun, Sci., 94, 1941, 520). 

 Cultural characters differ from those of 

 Pseudomonas aeruginosa Migula. 



Source: Repeatedly isolated from leaf 

 spot of tobacco in the Philippines. 



Habitat: Pathogenic on tobacco. 



93. Pseudomonas viridiflava (Burk- 

 holder, 1930) Clara, 1934. (Phytomonas viri- 

 diflava Burkholder, Cornell Agr. Exp. Sta. 

 Mem. 127, 1930, 63; Clara, Science, 75, 1934, 

 111.) 



vi.ri.di.fla'va. L. viridis green; L. flavus 

 yellow; M.L. adj. viridiflavus greenish 

 yellow. 



Description from Clara (Cornell Agr. 

 Exp. Sta. Mem. 139, 1934, 30). 



Rods 0.75 to 1.5 by 1.5 to 3.15 microns. 

 Motile with 1 or 2 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Beef -extract agar colonies: Grayish 

 white, margins corrugated, edges irregular. 



Broth: Turbid in 36 hours. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath.,3^, 1942, 601). 



Acid but no gas from glucose, fructose, 

 mannose, arabinose, xjdose, mannitol and 

 glycerol. Alkaline reaction from salts of 

 acetic, citric, malic, lactic and succinic 



