134 



ORDER I. PSEUDOMONADALES 



acids. Sucrose, lactose, maltose, raffinose, 

 salicin, and salts of formic and tartaric acids 

 not fermented. 



Starch: No hydrolj'sis. 



Growth in broth plus 5 per cent NaCl. 



Aerobic, facultative. 



Comment: A variety that does not grow 

 in Uschinsky's solution and that produces 

 colonies of an unusual shape has been iso- 

 lated from the stems and leaves of blighted 

 beans in Denmark. See Petersen (Tidsskr. 

 f. Planteavl., 38, 1932, 851). 



Source: Two cultures isolated from 

 spotted beans, one from England and one 

 from Switzerland. 



Habitat: Pathogenic on bean, Phaseolus 

 vulgaris. 



94. Pseudoitionas ananas Serrano, 1934. 

 (Philipp. Jour. Sci., 55, 1934, 355.) 



a'na.nas. Braz.Ind. ananas pineapple; 

 M.L. indecl.neut.noun ananas. 



Rods 0.6 by 1.8 microns. Motile with 1 to 

 4 polar flagella. Gram-negative. 



Green fluorescent pigment produced in 

 certain media. 



Gelatin: Liquefied. 



Beef -extract glucose agar colonies: White, 

 with undulating edges, smooth to rugose, 

 glistening to dull. 



Beef-extract agar: Growth scant. 



Broth: Feeble growth. 



Milk: Becomes alkaline with curd. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, xylose and 

 mannitol. Feeble with lactose. No acid from 

 sucrose. 



Starch not hydrolyzed. 



Temperature relations : Optimum between 

 30° and 31° C. Minimum between 7° and 

 10° C. Maximum, 45° C. 



Aerobic. 



Source: Isolated from rotted pineapples. 



Habitat: Causes a rot of pineapples. 

 Ananas comosus. 



95. Pseudomonas bowlesiae (Lewis and 



Watson, 1927) Dowson, 1943.* (Phytomonas 

 bowlesii (sic) Lewis and Watson, Phyto- 

 path., 17, 1927, 511; Pseudomonas bowlesiae 

 Dowson, Trans. Brit. Mycol. Soc, 26, 1943, 

 9.) 



bow.le'si.ae. M.L. fem.n. Bowlesia ge- 

 neric name; M.L. gen. noun bowlesiae of 

 Bowlesia. 



Rods, 0.5 to 0.7 by 1.2 to 1.6 microns, 

 occurring singly, in pairs or in short chains. 

 Motile with bipolar flagella. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Agar slants: Yellowish, moist, glistening 

 and viscid. 



Broth: Uniform turbidity throughout. 

 Heavy viscous sediment in old cultures. 



Milk: Alkaline; coagulation with a slow 

 peptonization. 



Nitrites produced from nitrates. 



Indole produced. 



Hj'drogen sulfide produced. 



Acid from glucose, maltose and xylose. 

 No acid from sucrose. 



Temperature relations: Optimum, 27° C. 

 Minimum, —1° C. Maximum, 37° C. 



Chemical tolerance: Optimum pH, 7.2. 

 pH range, 4.5 to 8.6. 



Aerobic. 



Source: Isolated from diseased, water- 

 soaked spots of bowlesia. 



Habitat: Pathogenic on Bowlesia septen- 

 trionalis. 



96. Pseudomonas ligustri (d'Oliveira, 

 1936) SSvulescu, 1947. {Bacterium ligustri 

 d'Oliveira, Revista Agron., 2J^, 1936, 434; 

 Savulescu, Anal. Acad. Romane, III, 22, 

 1947, 11.) 



li.gus'tri. L. ligustrum the privet; M.L. 

 neut.noun Ligustrum generic name of 

 privet; ligustri of the privet. 



Rods 0.5 to 0.7 by 1.3 to 3.0 microns. No 

 chains. Not encapsulated. Motile with 2 to 

 5 polar flagella. Gram-negative. 



Green pigment produced on Dox agar and 

 in broth. 



Gelatin: Liquefied. 



* The authors of this binomial report (personal communication) that the original spell- 

 ing bowlesii used for the specific epithet is an orthographic error. The correct spelling is 

 "bowlesiae" . 



