FAMILY IV. PSEUDOMONADACEAE 



135 



Beef -extract agar colonies: Growth mod- 

 erate. Milky white, circular, convex. 



Broth: Turbid in 24 hours. No pellicle. 



Milk: Coagulated in 6 days and later di- 

 gested. Litmus slightly acid. 



Nitrites not produced from nitrates. 



Indole not produced. 



Ammonia not produced. 



No gas from carbohydrates. Acid from 

 glucose, galactose, arabinose and mannose. 

 No acid from sucrose, maltose, lactose, raf- 

 finose, raannitol or salicin. 



Source: From diseased Japanese privet 

 in Lisbon, Portugal. 



Habitat: Pathogenic on privet, Ligus- 

 trum ja'ponicum. 



97. Pseudomonas marginalis (Brown, 

 1918) Stevens, 1925. {Bacterium marginale 

 Brown, Jour. Agr. Res., 13, 1918, 386; 

 Stevens, Plant Disease Fungi, New York, 

 1925, 30; Phyfomonas intyhi Swingle, Phyto- 

 path., 15, 1925, 730.) 



mar.gi.na'lis. L. margo, marginis edge, 

 margin; M.L. adj. marginalis marginal. 



Description from Brown (op. cit., 1918, 

 386) and Clara (Cornell Agr. Exp. Sta. Mem. 

 159, 1934, 27). 



Rods. Motile with 1 to 3 polar flagella. 

 Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Agar colonies: Cream-colored to yellow- 

 ish. 



Broth: Turbid, with pellicle. 



Milk: Alkaline. Soft curd at times. 



Nitrites are produced from nitrates. Not 

 produced (Clara). 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, galactose, 

 fructose, mannose, arabinose, xylose, rham- 

 nose, mannitol and glycerol. Alkali from 

 salts of acetic, citric, malic, formic, lactic, 

 succinic and tartaric acids. Sucrose, mal- 

 tose, lactose, raffinose and salicin not fer- 

 mented (Clara). 



Starch hydrolysis feeble. None (Clara). 



Temperature relations : Optimum between 

 25° and 26° C. Minimum, 0° C. Maximum, 

 38° C. 



Aerobic. 



Source: Isolated from marginal lesion on 

 lettuce from Kansas. 



Habitat: Pathogenic on lettuce and re- 

 lated plants. 



98. Pseudomonas sesami Malkoff, 1906. 

 (Cent. f. Bakt., II Abt., 16, 1906, 665.) 



se'sa.mi. Gr. sesamum sesame; M.L. 

 neut.noun Sesamum generic name of sesame; 

 sesami of sesame. 



Description from Nakata (Ann. Phyt. 

 Soc. Japan, .?, 1930, 242). 



Rods 0.6 to 0.8 by 1.2 to 3.8 microns. 

 Motile with 2 to 5 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefaction rapid. 



Beef -agar colonies: Circular, flat, striate, 

 smooth, entire margins, white. 



Broth: Growth rapid. No pellicle. 



Milk: Alkaline. No coagulation. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose. No acid 

 from lactose, sucrose or glycerol. 



Starch not hj^drolyzed. 



Temperature relations: Optimum, 30° C. 

 Minimum, 0° C. Maximum, 35° C. 



Aerobic, facultative. 



Source: Isolated from l)rown spots on 

 leaves and stems of sesame. 



Habitat: Pathogenic on sesame. 



99. Pseudomonas setariae (Okabe, 

 1934) Savulescu, 1947. (Bacterium setariae 

 Okabe, Jour. Soc. Trop. Agr. Formosa, 6, 

 1934, 63; SSvulescu, Anal. Acad. Romane, 

 III, 22, 1947, 11.) 



se.ta'ri.ae. L. saeta a bristle; M.L. 

 saetarius bristle-like; M.L. fem.noun Se- 

 taria generic name of foxtail; setariae of 

 Setaria. 



Rods 0.4 to 0.8 by 1.8 to 4.4 microns. 

 Motile with a polar, seldom bipolar, flagel- 

 lum. Gram-negative. 



Yellowish, water-soluble pigment pro- 

 duced in culture. 



Gelatin: Slow liquefaction. 



Beef -extract agar colonies: Circular, 

 white, opalescent, smooth, glistening. 



Broth: Tur])id after 18 hours. Pellicle. 



