136 



ORDER I. PSEUDOMONADALES 



Milk: Alkaline; clears. 



Nitrites produced from nitrates. 



Indole produced. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, galactose 

 and glycerol. No acid from lactose, maltose 

 or sucrose. 



Starch: Feeble hydrolysis. 



Grows in 3 per cent salt. 



Temperature relations: Optimum be- 

 tween 31° and 34° C. Maximum, 42° C. 



Aerobic. 



Source: Isolated from brown stripe of 

 Italian millet. 



Habitat: Pathogenic on Italian millet, 

 Setaria italica. 



100. Pseudomonas tolaasii Paine, 1919. 

 (Ann. Appl. Biol., 5, 1919, 210.) 



to.laa'si.i. Tolaas patronymic; tolaasii 

 of Tolaas. 



Rods 0.4 to 0.5 by 0.9 to 1.7 microns. 

 Motile with 1 to 5 polar flagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Bouillon agar: Streak develops in 24 

 hours, dirty bluish white, wet-shining and 

 slightly raised. 



Broth: Turbid in 24 hours. Pellicle. 



Milk: Becomes alkaline and clears. 



Nitrites not produced from nitrates. 



Indole production slight. 



Acid but no gas from glucose. No acid 

 from lactose or sucrose. 



Starch hydrolysis feeble. 



Optimum temperature, 25° C. 



Source : Isolated in England from brown- 

 spot of cultivated mushrooms. 



Habitat: Pathogenic on cultivated mush- 



101. Pseudomonas washingtoiiiae 



(Pine 1943) Elliott 1951. {Phytomonas 

 washingtoniae Pine, Phytopath., 33, 1943, 

 1203; Elliott, Man. Bact. Plant Path., 2nd 

 ed., 1951, 100.) 



wash.ing.to'ni.ae. M.L. fem.noun Wash- 

 ingtonia a generic name; washingtoniae of 

 Washingtonia. 



Rods, 0.69 l)y 1.61 microns, occurring 



singly or in short chains. Motile with 1 to 3 

 polar flagella. Gram-negative. 



Green pigment in certain media. 



Gelatin: Liquefied. 



Potato dextrose agar colonies: Circular, 

 smooth, convex, glistening, white to cream, 

 butyrous, edges entire. 



Milk: No curd; peptonization with a green 

 color in 7 days. 



Indole not produced. 



Nitrites not produced from nitrates. 



Hydrogen sulfide produced in minute 

 amounts. 



Acid but no gas from glucose, fructose 

 and L-arabinose in 24 hours; from galactose 

 and xylose in 48 hours. No acid from sucrose, 

 lactose, cellobiose, maltose, mannitol, 

 D-sorbitol, glycerol, salicin or raffinose. 



Starch not hydrolyzed. 



Aerobic. 



Thermal death point between 47° and 

 48° C. 



Source : Isolated from spots on the leaves 

 of the palm, Washingtonia filifera. 



Habitat: Pathogenic on the Washington 

 palm. 



102. Pseiidonioiias barker! (Berridge, 

 1924) Clara, 1934. (Bacillus of pear blossom 

 disease. Barker and Grove, Ann. Appl. 

 Biol., 1, 1914, 94; Barker and Grove's or- 

 ganism, Doidge, Ann. Appl. Biol., 4, 1917, 

 50; B. barkeri Berridge, Ann. Appl. Biol., 11, 

 1924, 73; Clara, Science, 75, 1934, 11.) 



bar'ker.i. M.L. gen. noun barkeri of 

 Barker; named for B. T. P. Barker, one of 

 the two men who first described this or- 

 ganism. 



Description from Doidge (op. cit., 1917, 

 50). 



Rods 0.5 to 0.8 by 2.0 to 4.0 microns. 

 Motile with 1 to 4 polar flagella. Gram-nega- 

 tive (Burkholder), not Gram-positive. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Liquefied. 



Agar: Growth is white, feeble, flat, glist- 

 ening, smooth-edged. 



Broth: Slightly turbid in 24 hours. 



Milk: Slowly cleared. 



Nitrites not produced from nitrates. 



Indole not produced unless culture 

 warmed. 



