FAMILY IV. PSEUDOMONADACEAE 



139 



Starch hydrolysis feeble. 



Temperature relations : Optimum between 

 27° and 28° C. Maximum, 37° C. 



Chemical tolerance: Optimum pH be- 

 tween 6.2 and 6.8. pH range, 5.4 to 8.9. 



Source: Isolations from naturally infected 

 timg oil trees in Georgia. 



Habitat: Pathogenic on the tung oil tree 

 (Aleurites Jordi), on the bean {Phaseolus 

 vulgaris) and the castor bean (Ricinus com- 

 munis) . 



110. Pseudoinonas glycinea Coerper, 

 1919. (Jour. Agr. Res., 18, 1919, 188.) 



gly.ci'ne.a. Gr. glycys sweet; M.L. 

 f em. noun Glycine generic name of a legume ; 

 M.L. adj. glycinea of the soybean. 



Rods 1.2 to 1.5 by 2.3 to 3.0 microns. 

 Motile with polar flagella. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: Not liquefied. 



Beef -peptone agar colonies: Appear in 

 24 hours. Circular, creamy white, smooth, 

 shining and convex. Margins entire. Buty- 

 rous in consistency. 



Milk: Litmus turns blue and later a sepa- 

 ration of the milk occurs. Casein not di- 

 gested. 



Nitrites produced from nitrates (Burk- 

 holder and Starr, Phytopath., 38, 1948, 498). 



Indole test feebly positive. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid from glucose and sucrose. 



Starch not hydrolyzed. 



Temperature relations: Optimum be- 

 tween 24° and 26° C. Minimum, 2° C. Maxi- 

 mum, 35° C. 



Aerobic, facultative. 



Comment: A variety of this species that 

 differs slightly in morphology, action in 

 milk and in chromogenesis has been de- 

 scribed by Takimoto (Jour. Plant Prot., 

 Tokyo, 14, 1927 556). It was isolated from 

 leaf spots on soy bean in Formosa. 



Source: A number of cultures isolated 

 from soybeans in Wisconsin. 



Habitat: Pathogenic on soybean. Glycine 

 max {Soja niax) . 



111. Pseudonionas savastanoi (Erw. 

 Smith, 1908) Stevens, 1913. (Bacterium 



savastanoi Erw. Smith, U. S. Dept. Agr. 

 Plant Ind. Bull. 131, 1908, 31; Stevens, The 

 Fungi which Cause Plant Diseases, 1913, 

 33.) 



sa.vas.ta'no.i. Savastano patronymic; 

 savastanoi of Savastano. 



Description from Brown (Jour. Agr. Res., 

 U, 1932, 711). 



Rods 0.4 to 0.8 by 1.2 to 3.3 microns. 

 Motile with 1 to 4 polar flagella. Gram- 

 negative. 



Green fluorescent pigment found in cul- 

 ture. 



Gelatin: No liquefaction. 



Beef agar colonies: White, smooth, flat, 

 glistening, margins erose or entire. 



Broth: Turbid on the second day. No 

 pellicle or ring. 



Milk: Becomes alkaline. 



Nitrites not produced from nitrates. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose, galactose 

 and sucrose. 



Starch hydrolyzed. 



Temperature relations: Optimum be- 

 tween 23° and 24° C. Minimum, 1° C. Max- 

 imum, 32° C. 



Chemical tolerance: Optimum between 

 6.8 and 7.0. Minimum, 5.6. Maximum, 8.5. 



Aerobic. 



Comment: A variety that differs but 

 slightly from this species is described as 

 pathogenic on ash, Fraxinus excelsior and F. 

 americana, but not on olive. Produces a 

 canker on ash. See Brown (Jour. Agr. Res., 

 U, 1932, 721). 



Source: Smith isolated his cultures from 

 olive galls collected in California. 



Habitat: Pathogenic on olive. 



112. Pseudoinonas tonelliana (Ferra- 

 ris, 1926) Burkholder, 1948. {Bacterixim to- 

 nellianum Ferraris, Trattato di Patologia e 

 TerapiaVegetale,3rded.,/, 1926, 104; Burk- 

 holder, in Manual, 6th ed., 1948, 132.) 



to.nel.li.a'na. M.L. adj. tonellianus per- 

 taining to Tonelli; named for A. Tonelli. 



Description from C. O. Smith (Phyto- 

 path., 18, 1928, 503) unless otherwise noted. 



Rods 0.5 to 0.6 by 1.5 to 2.5 microns. 

 Motile with 1 to 3 polar flagella. Gram- 

 negative (Adam and Pugsley, Jour. Dept. 

 Agr. Victoria, 32, 1934, 304). 



