140 



ORDER I. PSEUDOMONADALES 



Gelatin: No liquefaction. 



Potato glucose agar colonies: Flat, circu- 

 lar, shining; margins somewhat undulated. 



Broth: Dense clouding with partial pel- 

 licle. 



Milk: Alkaline. No separation. 



Nitrites not produced from nitrates 

 (Adam and Pugsley). 



Indole produced. Not produced (Adam 

 and Pugsley). 



Acid but no gas from glucose and sucrose. 

 No acid from lactose (Adam and Pugsley). 



Starch not hydrolyzed (Adam and Pugs- 

 ley). 



Comment: Pseudomonas savastanoi is 

 similar in culture but is not pathogenic on 

 oleanders. 



Source: Both Ferraris and C. O. Smith 

 isolated the pathogen from galls on olean- 

 der. 



Habitat: Pathogenic on oleander, Nerium 

 oleander. 



113. Pseudomonas cissicola (Takimoto, 

 1939) Burkholder, 1948. (Aplanobacter cissi- 

 cola Takimoto, Ann. Phytopath. Soc. Japan, 

 9, 1939, 43; Burkholder, in Manual, 6th ed., 

 1948, 134.) 



cis.si'co.la. Gr. cissus ivy; M.L. fem.noun 

 Cissiis generic name of flowering plant; 

 L. -cola dweller; M.L. fem.noun cissicola 

 Cissus dweller. 



Rods 0.5 to 0.9 by 1.0 to 2.0 microns. 

 Non-motile. Encapsulated. Gram-negative. 



Green fluorescent pigment formed in 

 Uschinsky's solution. 



Gelatin: No liquefaction. 



Potato-extract agar colonies: Circular, 

 conve.x, smooth, dirty white. 



Broth: Feeble clouding followed by pre- 

 cipitation of pellicle and rim. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



No acid nor gas from sucrose, glucose, lac- 

 tose or glycerol. 



Starch not hydrolyzed. 



Salt toleration, 3 per cent. 



Temperature relations: Optimum, 30° C. 

 Minimum, 10° C. Maximum, 35° C. Ther- 

 mal death point between 49° and 50° C. 



Source: Isolated from black spots on 



leaves of Japanese ivy, Cissus japonica, in 

 Japan. 



Habitat: Pathogenic only on Cissus ja- 

 ponica. 



114. Pseudomonas ealendulae (Taki- 

 moto, 1936) Dowson, 1943. (Bacterium 

 ealendulae Takimoto, Ann. Phytopath. Soc. 

 Japan, 5, 1936, 341; Dowson, Trans. Brit. 

 Mycol. Soc, 26, 1943, 9.) 



ca.len'du.lae. L. fem.pl. noun calendae 

 (Kalendae) The Calends, first day of month; 

 M.L. fem. dim. noun Calendula generic name 

 of a flowering plant; M.L. gen. noun ealen- 

 dulae of Calendula. 



Rods 0.5 by 1.0 to 2.0 microns. Motile 

 with 1 to 3 polar flagella. Gram-negative. 



Green fluorescent pigment produced in 

 Uschinsky's and in Cohn's solutions. 



Gelatin: Not liquefied. 



Agar colonies: Circular, smooth, flat, 

 dirty white. 



Broth: Turbid. 



Milk: No coagulation. 



Nitrites not produced from nitrates. 



Indole produced in small amount. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose and glycerol. 

 No acid from lactose or sucrose. 



Starch not hydrolyzed. 



Temperature relations: Optimum be- 

 tween 27° and 30° C. Minimum between 0° 

 and 7° C. Maximum, 37° C. 



Habitat: Pathogenic on marigolds, Calen- 

 dula officinalis. 



115. Pseudomonas cichorii (Swingle, 

 1925) Stapp, 1928. {Phjtomonas cichorii 

 Swingle, Phytopath., 15, 1925, 730; Stapp, in 

 Sorauer, Handbuch der Pfianzenkrank- 

 heiten, 2, 5 Aufll., 1928, 291; Pseudomonas 

 endiviae Kotte, Phyt. Ztschr., 1, 1930, 609; 

 Bacterium formosanum Okabe, Jour. Soc. 

 Trop. Agr., Formosa, 7, 1935, 65.) 



ci.cho'ri.i. Gr. cichora (pi.) succory, chi- 

 cory; L. cichoriutn chicory; M.L. neut.noun 

 Cichoriutn generic name of flowering plant; 

 M.L. gen. noun cichorii of chicory. 



Description from Clara (Cornell Agr. 

 Exp. Sta. Mem. 159, 1934, 26) which is a de- 

 scription of a culture of Pseudomonas endi- 

 viae from Kotte. Swingle's description is 

 very meager. 



