FAMILY IV. PSEUDOMONADACEAE 



141 



Rods 0.75 to 1.5 by 1.5 to 3.75 microns. 

 Motile with 1 or 2 polar fiagella. Gram- 

 negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: No liquefaction. 



Beef -extract agar colonies: Circular, 

 grayish white with bluish tinge, raised with 

 slightly irregular edges. 



Broth: Turbid in 36 hours with a smooth, 

 viscous pellicle. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not produced. 



H3'drogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, Phj^- 

 topath., 32, 1942, 601). 



Acid but no gas from glucose, galactose 

 fructose, mannose, arabinose, xylose, man- 

 nitol and glycerol. Alkaline production from 

 salts of acetic, citric, lactic, malic, succinic 

 and tartaric acids. Rhamnose, maltose, 

 sucrose, lactose, raffinose and salicin not 

 utilized. 



Starch not hydrolyzed. 



Slight growth in broth plus 6 per cent 

 NaCl. 



Chemical tolerance: Optimum pH, be- 

 tween 6.8 and 7.1. Minimum, between 5.0 

 and 5.3. Maximum, between 9.2 and 9.4. 

 (Kotte, op. cit., 2, 1930, 453). 



Aerobic, facultative. 



Source: Isolated from rot of French en- 

 dive, Cichorium intybus, by Swingle and by 

 Okabe, and from C. endivia by Kotte. 



Habitat: Pathogenic on endive, lettuce 

 and larkspur. 



116. Pseiidomonas nectarophila 



(Doidge, 1917) Rosen and Bleeker, 1933. 

 {Bacterium nectar ophilum Doidge, Ann. 

 Appl. Biol., J^, 1917, 73; Rosen and Bleeker, 

 Jour. Agr. Res., ^6, 1933, 98.) 



nee. ta.ro 'phi. la. Gr. nectar nectar; Gr. 

 adj. philus loving; M.L. adj. nectarophilns 

 nectar-loving. 



Rods 0.5 to 0.7 by 0.6 to 1.5 microns. 

 Motile with 1 to 5 polar flagella. Encapsu- 

 lated. Gram-negative. 



Green fluorescent pigment produced in 

 culture. 



Gelatin: No liquefaction. 



Nutrient agar colonies: Yellowish white, 

 wet-shining, smooth; margins irregular. 



Broth: Heavy turbidity in 24 hours. 

 Sediment. 



Milk: Cleared. 



Nitrites not produced from nitrates. 



Indole not produced. 



Acid from glucose and galactose. No acid 

 from sucrose. 



Starch hydrolysis feeble. 



Optimum temperature between 25° and 

 30° C. 



Aerobic, facultative. 



Distinctive character : Differs from Pseu- 

 domonas barkeri in that it does not liquefy 

 gelatin nor produce indole. Produces cap- 

 sules. 



Source : Isolated from blighted pear blos- 

 soms in South Africa. 



Habitat: Pathogenic on pear blossoms. 



117. Pseudomonas viburni (Thornberry 

 and Anderson, 1931) Stapp, 1935. (Phyto- 

 monas viburni Thornberry and Anderson, 

 Phytopath., 21, 1931, 912; Stapp, Bot. Rev. 

 1, 1935,407.) 



vi.bur'ni. L. viburnum waj' -faring tree; 

 M.L. neut.noun Viburnum name of a genus 

 of flowering plants; M.L. gen. noun viburni 

 of Viburnum. 



Rods 0.5 to 1.0 by 1.0 to 2.0 microns. 

 Encapsulated. Motile with 2 to 4 polar 

 flagella. Gram-negative (Burkholder); not 

 Gram-positive as stated in original. 



Green fluorescent pigment produced in 

 culture (Burkholder). 



Gelatin: No liquefaction. 



Glucose beef-extract colonies: Dull gray, 

 circular, edges entire. 



Broth: Turbid with pellicle. 



Milk: Alkaline. 



Nitrites not produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Not lipolytic (Starr and Burkholder, 

 Phytopath., 32, 1942, 601). 



Acid from glucose and galactose but not 

 sucrose (Burkholder). 



Starch: No hydrolysis. 



Slight growth in 3.5 per cent salt (Burk- 

 holder). 



Temperature relations: Optimum, 25° C. 

 Minimum, 12° C. Maximum, 35° C. 



