FAMILY IV. PSEUDOMONADACEAE 



143 



(Phytomonas alliicola Burkholder, Phyto- 

 path., 32, 1942, 146; Starr and Burkholder, 

 Phytopath., ibid., 601.) 



al.li.i'co.la. L. allium onion; L. -cola 

 dweller; M.L. fern. noun alliicola onion 

 dweller. 



Rods 0.7 to 1.4 by 1.05 to 2.8 microns. 

 Motile with 1 to several polar flagella, at 

 times bipolar. Gram-negative. 



Gelatin: Liquefied. 



Beef -extract peptone agar streaks : Mod- 

 erate in growth, white at first, later dirty 

 in appearance, edges wavy, consistency vis- 

 cid. Medium deep brown. 



Potato-glucose agar frequently becomes 

 greenish. 



Broth: Turbid with light pellicle. Brown. 



Milk: Cleared and litmus reduced. Neu- 

 tral. 



Nitrites produced from nitrates. 



Indole not produced. 



Hydrogen sulfide not produced. 



Lipolytic action very strong. 



Acid but no gas from 1-arabinose, d-xy- 

 lose, rhamnose, glucose, d-galactose, fruc- 

 tose, d-lactose, maltose, sucrose, glycerol, 

 mannitol and salicin. Alkali from salts of 

 acetic, citric, formic, hippuric, lactic, malic, 

 succinic and tartaric acids. 



Starch not hydrolyzed. 



Slight growth in broth plus 4 per cent 

 salt. 



Aerobic. 



Temperature relations: Optimimi, 30° C. 

 Minimum, 5° C. Maximum, 41° C. 



Source: Seven isolates from storage rot 

 of onion bulbs. 



Habitat: Pathogenic on onion bulbs, Al- 

 lixun cepa. 



122. Pseiidonionas gardeniae Burk- 

 holder and Pirone, 1941. (Phytopath., 31, 

 1941, 194.) 



gar.de'ni.ae. L. Garden patronymic; 

 M.L. fem.noun Gardenia plant generic 

 name; M.L. gen. noun gardeniae of Gardenia. 



Rods 0.75 by 2.4 microns. Motile with 

 1 to 2 polar flagella. Gram-negative. 



Gelatin: Liquefied. 



Beef -extract peptone agar colonies: 

 Growth fair, white to dirty gray and viscid. 

 Medium becoming dark brown. 



Potato-glucose agar: No brown color. 



Broth: Turbid with pellicle. Dark brown. 



Milk: Soft curd with pellicle. Clears in 

 zones. Litmus reduced. 



Nitrites produced from nitrates. 



Hydrogen sulfide not produced. 



Indole not produced. 



Acid from glucose, galactose, xylose, 

 rhamnose, sucrose, maltose, mannitol, 

 glycerol and salicin. Alkali produced from 

 the salts of citric, malic, malonic, succinic, 

 tartaric and hippuric acids. Good growth 

 in tyrosine and in asparagine broth. 



Starch not hydrolyzed. 



Aerobic. 



Source: Eight isolates from leaf spots of 

 gardenias in New Jersey. 



Habitat : Pathogenic on leaves of Gardenia 

 jasminoides. 



123. Pseudonionas caryophylli (Burk- 

 holder, 1942) Starr and Burkholder, 1942. 

 {Phytomonas caryophylli Burkholder, Phy- 

 topath., 32, 1942, 143; Starr and Burk- 

 holder, ibid., 601.) 



ca.ry.o'phyl.li. Gr. caryophyllum nut 

 leaf, the clover tree; M.L. mas. noun 

 caryophyllus specific epithet in Dianthus 

 caryophyllus , the clove-pink or carnation; 

 M.L. gen. noun caryophylli of the carnation. 



Rods 0.35 to 0.95 by 1.05 to 3.18 microns. 

 At times slightly curved. Motile with 1 to 

 several polar flagella. Frequently bipolar. 

 Gram-negative. 



Gelatin: Liquefaction after 3 to 4 weeks. 



Potato glucose agar colonies: 3 to 4 mm in 

 diameter, circular, smooth, glistening, 

 edges entire. Color is tan to gray-mauve. 

 Old culture dark brown. Consistency buty- 

 rous. 



Broth: Turbid with a white sediment. 



Milk: Litmus slowly becomes blue. Slight 

 reduction at bottom of tube. No clearing. 



Nitrites produced from nitrates. Also 

 ammonia and gas are produced in a syn- 

 thetic nitrate medium. Asparagine, KNO3 

 and NH4H2PO4 can be utilized. 



Indole not produced. 



Hydrogen sulfide not produced. 



Lipolytic action slight to moderate. 



Acid from 1-arabinose, d-xylose, rham- 

 nose, glucose, d-galactose, fructose, d-lac- 

 tose, maltose, sucrose, glj^cerol, mannitol 

 and salicin. Alkali with sodium salts of 



