FAMILY IV. PSEUDOMO^^ADACEAE 



145 



Motile with 1 to 5 polar flagella. Encapsu- 

 lated. Gram-negative. 



Gelatin: Liquefied. 



Beef -peptone agar colonies: Small, flat, 

 .smooth, dry, shining, translucent, graj^ish 

 and butyrous. 



Broth: Turbid in 4 days. Transient pel- 

 licle. 



Milk: Slightly alkaline. No coagulation 

 nor clearing. 



Nitrites not produced from nitrates. No 

 growth on synthetic nitrate agar. 



Indole not produced. 



Hydrogen sulfide not produced. 



Acid reaction occurs in galactose, starch 

 and sucrose. No gas. 



Starch not hj-drolyzed. 



Source : From diseased leaves and fruit of 

 the passion fruit in New Zealand. 



Habitat: Pathogenic on Passiflora edulis. 



127. Pseudomonas seininuni Caylej', 

 1917. (Jour. Agr. Sci., 8, 1917, 461.) 



se'mi.num. L. semen, sennnis seed; L. 

 gen.pl. semimim of seeds. 



Rods 1.0 by 4.0 to 5.0 microns. Spore- 

 like bodies present. Encapsulated. Motile 

 with a single flagellum. Reported as Gram- 

 po.sitive; however, probablj^ in error. No 

 cultures are available for a retest of this 

 character. 



Gelatin: Rapid liquefaction. 



Agar colonies: White, more or less circu- 

 lar, transparent, spreading. 



Broth: Turbid. Pellicle. 



Litmus milk: Milk becomes clear and 

 apricot color. 



Nitrites produced from nitrates. 



Acid but no gas from glucose and sucrose. 

 No acid from lactose. 



Starch: No hydrolysis. 



Optimum temperature, 25° C. 



Aerobic, facultative. 



Source: Isolated from seeds, stems and 

 pods of diseased peas in England. 



128. P.seiidonionas vitiswoodrowii Pa- 

 id and Kulkarni, 1951. (Pseudomonas ritis- 

 woodrowii (sic) Patel and Kulkai'ui, Curr. 

 Sci.,^0, 1951, 132.) 



vi.tis.wood.ro'wi.i. L. fem.noun vitis 

 a vine; M.L. fem.noun Vitis a generic name; 



Woodrow patronymic; M.L. gen. noun woo(/- 

 rowii of Woodrow. 



Rods 0.8 by 1.5 microns. Motile with 

 a single polar flagellum. Gram-negative. 



Gelatin: Liquefied. 



Potato dextrose agar colonies: Circular, 

 capitate with margins entire. Pale, dull 

 gray. 1.2 cm in diameter in 7 daj's. 



Broth: Turbid. 



Potato cylinders: Scant growth. Medium 

 dark gray. 



Milk : Litmus reduced and casein digested. 



Hydrogen sulfide produced. 



Loefiler's blood serum: Liquefied. 



Indole not produced. 



Synthetic asparagine medium. No growth. 



Methyl red test negative; acetylmethyl- 

 carbinol not produced. 



Nitrites not produced from nitrates. 



Acid but no gas from glucose, lactose and 

 sucrose, but no growth in salicin. 



Salt tolerance: Up to 1 per cent. 



Optimum temperature between 25° and 

 28° C. 



Aerobic. 



Source: Isolated from leaves of Vitis 

 woodrowii in India. 



Habitat : Pathogenic on V. woodrowii but 

 not on V. vinifera. 



129. Pseudomonas fabae (Yu, 1936) 

 Burkholder, 1948. (Phytomonas fabae Yu, 

 Bull, of the Chinese Bot. Soc, 2, 1936, 34; 

 Burkholder, in Manual, 6th ed., 1948, 139.) 



fa'bae. L. faba the horse bean; M.L. 

 gen. noun /aftoe of the hor.se bean. 



Rods 0.8 to 1.1 by 1.1 to 2.8 microns. Mo- 

 tile with 1 to 4 polar flagella. Gram-nega- 

 tive. 



Gelatin: Liquefied. 



Nutrient agar colonies: Circular, entire, 

 viscid, glistening, raised, smooth to 

 wrinkled, white to salmon. Medium amber. 



Broth: Turbid after 12 hours. Pellicle. 



Milk: Growth slow. Clears. 



Nitrites produced from nitrates. 



Indole production slight. 



Hydrogen sulfide not produced. 



Acid but no gas from glucose. No acid 

 nor gas developed from arabinose, xylose, 

 fructose, galactose, sucrose, lactose, mal- 

 tose, raffinose, dextrin, inulin, mannitol or 



